1. Example usage of mockrobiota MC resource for marker gene and metagenome sequencing pipelines.
Example usage of mockrobiota MC resource for marker gene and metagenome sequencing pipelines. MC data sets are selected on the basis of multiple input criteria, including data set metadata, sample metadata, and represented taxa. Raw data (e.g., fastq) are demultiplexed, sequences are dereplicated or clustered as operational taxonomic units (OTUs) (marker gene data) or assembled/scaffolded to template genomes (metagenome data), and representative sequences are annotated (e.g., by taxonomy or gene). Observed taxonomic/gene annotations and abundances are compared to the expected composition (expected taxonomic assignments/gene annotations and abundances) of that MC, e.g., to generate precision and recall scores or correlations between observed and expected values. QC, quality control.
Nicholas A. Bokulich et al. mSystems 2016; doi: /mSystems