1. Quantification of Circulating miRNAs in Plasma
Ioanna S. Sourvinou, Athina Markou, Evi S. Lianidou 
The Journal of Molecular Diagnostics 
Volume 15, Issue 6, Pages (November 2013)
DOI: /j.jmoldx
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

2. Figure 1
Isolation of plasma circulating miRNAs using TRIzol LS reagent. Raw Cq values for endogenous hsa-miR-21 after total RNA extraction, exogenous cel-miR-39 added in sample before total RNA extraction, and exogenous cel-miR-39 recovered after extraction are shown starting from different plasma volumes (A) and as the effect of temperature and time of the precipitation step (B). The data represent the means ± SD of three independent experiments. Statistical evaluation was based on the U-test. ∗P < 0.05.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

3. Figure 2
A: RT-qPCR raw Cq values for endogenous hsa-miR-21 after total RNA extraction, exogenous cel-miR-39 added in sample before total RNA extraction, and exogenous cel-miR-39 recovered after extraction from plasma samples using different commercially available extraction protocols. B: Recovery rates for the exogenous spiked-in control cel-miR-39. The data represent the means ± SD of three independent experiments. Statistical evaluation was based on the U-test. ∗P < 0.05.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

4. Figure 3
Stability study of circulating miRNAs in plasma at different storage time and temperature conditions. A and B: cel-miR-39. C and D: hsa-miR-21. E and F: hsa-miR-16. The results are presented as differences in raw Cq values from the 0-hour control (0h) (positive differences represent decreases and negative differences represent increases in miRNA concentrations) and as percentage recoveries from the 0h for each miRNA. The data represent the means ± SD of three independent experiments. Statistical evaluation was based on the U-test. ∗P < 0.05.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

5. Figure 4
Percentage recovery for exogenous cel-miR-39 before and after storage of extracted miRNAs for 2, 6, 8, 9, and 12 months. The data represent the means of three independent measurements.
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions

6. Figure 5
Effects of different normalization procedures on the quantification of circulating miRNA levels in the plasma of healthy individuals (n = 30) and patients with NSCLC (n = 30). The concentration of hsa-miR-21 in plasma was quantified by normalizing with respect to hsa-miR-16 (A), exogenous control cel-miR-39 (B), and a combination of both (C). ∗∗∗P <
The Journal of Molecular Diagnostics  , DOI: ( /j.jmoldx )
Copyright © 2013 American Society for Investigative Pathology and the Association for Molecular Pathology Terms and Conditions