1. Terpinen-4-ol, The Main Component of Melaleuca Alternifolia (Tea Tree) Oil Inhibits the In Vitro Growth of Human Melanoma Cells 
Annarica Calcabrini, Annarita Stringaro, Laura Toccacieli, Stefania Meschini, Manuela Marra, Marisa Colone, Giuseppe Arancia, Agnese Molinari 
Journal of Investigative Dermatology 
Volume 122, Issue 2, Pages (February 2004)
DOI: /j X x
Copyright © 2004 The Society for Investigative Dermatology, Inc Terms and Conditions

2. Figure 1
Expression of P-gp on human melanoma M14 sensitive and drug-selected cells. (a, b) Flow cytometric determination of surface P-gp in human melanoma M14 WT (a) and M14 ADR (b) cells labeled with MoAb MM4.17 (black histograms) or mouse isotypic globulins (negative control, dotted histograms). (c) Western blot of cell lysates from M14 WT (1) and M14 ADR (2) cells. The membrane was probed with the primary MoAb to P-gp (MoAb C219) and to actin (MoAb 1501). (d) Detection of MDR1 mRNA. PCR amplification products (40 cycles) fractioned by 2% agarose gel and visualized by ethidium bromide for human breast carcinoma MCF7-DX (positive control) (2), human melanoma M14 WT (3), and M14 ADR (4) cells. (1) DNA marker.
Journal of Investigative Dermatology  , DOI: ( /j X x)
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3. Figure 2
(a) Growth curves of human melanoma M14 WT cells grown in the absence (ctr) and in the presence of different concentrations of TTO. (b) Growth curves of human melanoma M14 ADR cells grown in the absence (ctr) and in the presence of different concentrations of TTO. Mean values and SD from three different experiments.
Journal of Investigative Dermatology  , DOI: ( /j X x)
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4. Figure 3
Apoptosis induced in human melanoma M14 WT (white columns) and M14 ADR (black columns) cells by different concentrations of TTO and after different exposure times. The AI was expressed as the percentage of cells binding annexin V but negative for PI. Mean values and SD from three different experiments.
Journal of Investigative Dermatology  , DOI: ( /j X x)
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5. Figure 4
Apoptosis induced in human melanoma M14 WT (black columns) and M14 ADR (gray columns) cells by serum starvation. In white columns AI of relative control cultures are reported. The AI was expressed as the percentage of cells binding annexin V but negative for PI. Mean values and SD from three different experiments.
Journal of Investigative Dermatology  , DOI: ( /j X x)
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6. Figure 5
Apoptosis induced in human melanoma M14 WT (black columns) and M14 ADR (gray columns) cells by the treatment with MoAb CH11 (25μg per mL) for 24 h. In white columns AI of relative control cultures are reported. The AI was expressed as the percentage of cells binding annexin V but negative for PI. Mean values and SD from three different experiments.
Journal of Investigative Dermatology  , DOI: ( /j X x)
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7. Figure 6
Apoptosis induced in sensitive (M14 WT) and resistant (M14 ADR) melanoma cells by treatment with terpinen-4-ol in the presence or absence of CsA. Mean values and SD from three different experiments.
Journal of Investigative Dermatology  , DOI: ( /j X x)
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8. Figure 7
Percent apoptosis (a, c) and cell survival (b, d) in sensitive (M14 WT) and resistant (M14 ADR) melanoma cells by treatment with TTO (a, b) or terpinen-4-ol (c, d) in the presence or absence of caspase inhibitors. Mean values and SD from three different experiments.
Journal of Investigative Dermatology  , DOI: ( /j X x)
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9. Figure 8
Scanning electron microscopy of human melanoma M14 WT cell cultures. (a) Control cells. Cells treated with (b) 0.005% TTO, (c) 0.01% TTO, and (d) 0.02% TTO.
Journal of Investigative Dermatology  , DOI: ( /j X x)
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10. Figure 9
Scanning electron microscopy of human melanoma M14 ADR cell cultures. (a) Control cells. Cells treated with (b) 0.005% TTO, (c) 0.01% TTO, and (d) 0.02% TTO.
Journal of Investigative Dermatology  , DOI: ( /j X x)
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11. Figure 10
Scanning electron microscopy of human melanoma M14 WT (a, b) and M14 ADR cells (c, d) treated with 0.01% terpinen-4-ol for 48 h..
Journal of Investigative Dermatology  , DOI: ( /j X x)
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12. Figure 11
Plasma membrane of FF human melanoma M14 cells. (a) Exoplasmic fracture face of a M14 WT cell. (b) Protoplasmic fracture face of a M14 ADR cell.
Journal of Investigative Dermatology  , DOI: ( /j X x)
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13. Figure 12
FF human melanoma M14 ADR cells. (a) Cross-fracture of a cell treated with 0.02% TTO, displaying numerous polarized blebs. Membrane blebs displayed both clustered intramembrane particles (b) and smooth lipid areas (c).
Journal of Investigative Dermatology  , DOI: ( /j X x)
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14. Figure 13
Plasma membrane of FF human melanoma M14 ADR cells. Lipid domains of about 200 to 250 nm were detectable on the protoplasmic fracture face (a, b). These smooth lipid domains tended to extrude from the plasma membrane (b).
Journal of Investigative Dermatology  , DOI: ( /j X x)
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