1. NUT Midline Carcinoma: An Aggressive Intrathoracic Neoplasm
Sameer A. Parikh, MD, Christopher A. French, MD, Brian A. Costello, MD, Randolph S. Marks, MD, Roxana S. Dronca, MD, Craig L. Nerby, MD, Anja C. Roden, MD, Vijay G. Peddareddigari, MD, John Hilton, MD, Geoffrey I. Shapiro, MD, Julian R. Molina, MD, PhD 
Journal of Thoracic Oncology 
Volume 8, Issue 10, Pages (October 2013)
DOI: /JTO.0b013e3182a00f41
Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

2. FIGURE 1
A, Computed tomography scan of the chest showing a 6 × 6cm mass in the right hilar area and an enlarged subcarinal lymph node. B, Pictorial depiction of the typical breakpoint in NUT midline carcinoma. NUT is present on 15q14 and BRD4 (bromodomain containing 4) on 19p13.1. Scattered neoplastic cells with large hyperchromatic nuclei, prominent nucleoli, and scant cytoplasm in a background of extensive necrosis (cell-block preparation, hematoxylin and eosin 40×).
Journal of Thoracic Oncology 2013 8, DOI: ( /JTO.0b013e3182a00f41)
Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

3. FIGURE 2
A, Computed tomography scan of the chest 2 months later showing an increase in the size of the right hilar mass and the subcarinal lymph node. B, Repeat biopsy 2 months later showing sheets of large neoplastic cells with vesicular chromatin and prominent nucleoli. Individual tumor cell necrosis and apoptosis is also seen (hematoxylin and eosin 40×). C, The neoplastic cells focally express keratin.
Journal of Thoracic Oncology 2013 8, DOI: ( /JTO.0b013e3182a00f41)
Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions

4. FIGURE 3
A, Nuclear protein in testis immunostain demonstrating a speckled pattern. B, FISH demonstrating BRD4-NUT translocation. Shown is dual color bring-together FISH on tumor cell nuclei using red and green probes covering BRD4 and NUT genes, respectively. Nuclei with red-green doublets demonstrate fusion of BRD4 and NUT. The nucleus with two red and two green signals represent nontumor nuclei containing two normal alleles each of BRD4 and NUT. FISH, fluorescent in situ hybridization; BRD4,; NUT, nuclear protein in testis.
Journal of Thoracic Oncology 2013 8, DOI: ( /JTO.0b013e3182a00f41)
Copyright © 2013 International Association for the Study of Lung Cancer Terms and Conditions