1. Lecture 5 Slides rh

2. Things to do
with glucose…
fig 14-1

3. adenosine triphosphate… ATP

4. fig 13-11

5. free energies are additive!
11kg
68kg
In metabolism,
it is all about the
coupling mechanism
riddle: how do you lift 11 kg?
ans: lower a weight > 11kg!

6. fig 13-9

7. It takes
ATP to
make
ATP!
the preparatory phase
fig 14-2

8. delayed gratification in glycolytic catabolism the payoff phase
fig 14-2

9. Glucose catabolism
around the bios…
fig 14-3

10. Phosphorylation of glucose
pg 532

11. Hexokinse: an example of induced fit
fig 6-22

12. Hexokinse: an example of induced fit
fig 6-22

13. Isomerization of glucose 6-P
pg 532

14. Anomers… rh

15. The structural simplicity of isomerase reactionrh

16. Phosphorylation of fructose 6-P
pg 532

17. Cleavage of fructose 1,6 bisphosphate by aldolase
pg 533

18. Cleavage of fructose 1,6 bisphosphate by aldolase
now have
two distinct
3-carbon
molecules
fig 14-6

19. DHAP isomerization gives us 2 identical G3P
pg 534

20. Isomerase action is familiar…
same chemistry, different enzyme

21. Equivalent carbons from different parts of glucose
fig 14-6

22. A fancy redox reaction…
pg 535

23. A fancy redox reaction catalyzed by GAPDH
fig 14-7

24. The GAPDH active site
fig 14-7

25. Enyzme-substrate complex
fig 14-7

26. a covalent intermediate between a cysteine S and the substrate
fig 14-7

27. The carbon
molecule
gets
oxidized!!
fig 14-7

28. Phosphate as a nucleophile!!??
fig 14-7

29. release
of the
product,
1,3 BPG
fig 14-7

30. Ready for more substrate…
fig 14-7

31. This is the product of interest!
fig 14-7

32. Voila!
Our
first
ATP! XX
pg 537

33. Substrate level phosphorylation: the general idea

34. “Isomerization” of 3PG to make 2PG
“mutase” is isomerase that moves groups
pg 537

35. Making an enol form by removal of H2O
pg 538

36. A second
substrate
level
phosphorylation:
more ATP!
pg 538

37. Ta Da!!! Pyruvic acid
pg 538

38. LDH converts
pyruvate to lactate,
restoring the pool
of NAD+
pg 547

39. Glycolysis: energy without O2
neutrophil
anaerobic
organisms
anaerobic
situations

41. CEOLACANTH: the Living Fossil

42. Glycolysis in action: 100 mt sprint
Usain Bolt!!