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Mucosal flora in inflammatory bowel disease

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Presentation on theme: "Mucosal flora in inflammatory bowel disease"— Presentation transcript:

1 Mucosal flora in inflammatory bowel disease
Alexander Swidsinski, Axel Ladhoff, Annelie Pernthaler, Sonja Swidsinski, Vera Loening–Baucke, Marianne Ortner, Jutta Weber, Uwe Hoffmann, Stefan Schreiber, Manfred Dietel, Herbert Lochs  Gastroenterology  Volume 122, Issue 1, Pages (January 2002) DOI: /gast Copyright © 2002 American Gastroenterological Association Terms and Conditions

2 Fig. 1 Percent of patients with high concentrations of mucosal bacteria. Gastroenterology  , 44-54DOI: ( /gast ) Copyright © 2002 American Gastroenterological Association Terms and Conditions

3 Fig. 2 (A) Adenine sequences of 12 clones from a patient with a predominance of an E. coli sequence. (B) Adenine sequences from a patient with a mix of different bacterial sequences. Gastroenterology  , 44-54DOI: ( /gast ) Copyright © 2002 American Gastroenterological Association Terms and Conditions

4 Fig. 3 FISH. Superficial bacterial layer (arrows) in unwashed and corresponding washed biopsies visualized with FISH using a universal probe. BL, basal lamina; G, goblet cell; N, nucleus. (A) CD patient with concentration of mucosal bacteria of 300 cfu/μL. (B) UC patient with concentration of mucosal bacteria of 1500 cfu/μL (B1) unwashed and (B2) washed. (C) CD patient with concentration of mucosal bacteria of 72,000 cfu/μL (C1) unwashed and (C2) washed. Gastroenterology  , 44-54DOI: ( /gast ) Copyright © 2002 American Gastroenterological Association Terms and Conditions

5 Fig. 4 Intracellular bacteria (arrows) visualized with Bacteroides-specific probe. G, goblet cell; N, nucleus. Gastroenterology  , 44-54DOI: ( /gast ) Copyright © 2002 American Gastroenterological Association Terms and Conditions

6 Fig. 5 Electron microscopic appearance of intraepithelial bacterial inclusions (arrows). (A-D) Concentrations of mucosal bacteria greater than 50,000 cfu/μL. (E) Concentration of mucosal bacteria of 19,000 cfu/μL. Bars = 1 μm. BL, basal lamina; er, endoplasmic reticulum; F, pericryptal fibroblast sheet; G, goblet cell; gr, mucous granules; M, mitochondria; N, nucleus; nu, nucleolus. Gastroenterology  , 44-54DOI: ( /gast ) Copyright © 2002 American Gastroenterological Association Terms and Conditions


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