Presentation is loading. Please wait.

Presentation is loading. Please wait.

R. Murata, M. D. , K. Nakagawa, M. D. , Ph. D. , S. Ohtori, M. D. , Ph

Similar presentations


Presentation on theme: "R. Murata, M. D. , K. Nakagawa, M. D. , Ph. D. , S. Ohtori, M. D. , Ph"— Presentation transcript:

1 The effects of radial shock waves on gene transfer in rabbit chondrocytes in vitro 
R. Murata, M.D., K. Nakagawa, M.D., Ph.D., S. Ohtori, M.D., Ph.D., N. Ochiai, M.D., Ph.D., M. Arai, M.E., T. Saisu, M.D., Ph.D., T. Sasho, M.D., Ph.D., K. Takahashi, M.D., Ph.D., H. Moriya, M.D., Ph.D.  Osteoarthritis and Cartilage  Volume 15, Issue 11, Pages (November 2007) DOI: /j.joca Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

2 Fig. 1 The relationship of pressure amplitude and relative luciferase activity (A) and cell viability (B). The cells were exposed to radial shock waves at 500 pulses and a frequency of 10Hz. Mean+SE (n=6), *P<0.05. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

3 Fig. 2 The relationship of number of pulses and relative luciferase activity (A) and cell viability (B). The cells were exposed to radial shock waves at an amplitude of 0.3MPa and a frequency of 10Hz. Mean+SE (n=6), *P<0.05. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

4 Fig. 3 The relationship of frequency and relative luciferase activity (A) and cell viability (B). The cells were exposed to radial shock waves at an amplitude of 0.3MPa and 500 pulses. Mean+SE (n=6), *P<0.05. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

5 Fig. 4 The relationship of DNA concentration and relative luciferase activity (A) and cell viability (B). The cells were exposed to radial shock waves at an amplitude of 0.3MPa, 2000 pulses, and a frequency of 10Hz. Mean+SE (n=6), *P<0.05. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions

6 Fig. 5 Flow cytometric analysis of GFP expression in transfected cells by radial shock waves or FuGENE6 methods. The cells treated by radial shock waves (RSW group: A) and FuGENE6 (FuGENE6 group: B) are shown. The dot plot of each group is shown as fluorescence of GFP (horizontal axis) and forward scatter (vertical axis). The cells within the sphere enclosed with a kinked line were analyzed and displayed in the histograms, respectively. In each histogram, the horizontal axis shows relative fluorescent intensity and the vertical axis shows cell numbers. Osteoarthritis and Cartilage  , DOI: ( /j.joca ) Copyright © 2007 Osteoarthritis Research Society International Terms and Conditions


Download ppt "R. Murata, M. D. , K. Nakagawa, M. D. , Ph. D. , S. Ohtori, M. D. , Ph"

Similar presentations


Ads by Google