1. Volume 107, Issue 12, Pages 2850-2859 (December 2014)
Biophysical Properties of Novel 1-Deoxy-(Dihydro)ceramides Occurring in Mammalian Cells 
Noemi Jiménez-Rojo, Jesús Sot, Jon V. Busto, Walt A. Shaw, Jingjing Duan, Alfred H. Merrill, Alicia Alonso, Félix M. Goñi 
Biophysical Journal 
Volume 107, Issue 12, Pages (December 2014)
DOI: /j.bpj
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2. Chart 1 Structures of the Cers studied in this work.
Biophysical Journal  , DOI: ( /j.bpj )
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3. Figure 1
Quantitation of the different Cer subtypes of RAW264.7 cells, showing the amounts of the N-acyl derivatives of the sphingoid-base backbones (d18:1, sphingosine; d18:0, sphinganine; m18:1, 1-deoxysphingosine; m18:0, 1-deoxysphinganine; m17:1, 1-deoxymethylsphingosine; and m17:0, 1-deoxymethylsphinganine) analyzed in RAW264.7 cells. In A and B, the y axis represents the sum of all of the N-acyl derivatives of these backbones (e.g., the bar for d18:1 represents all of the Cers with a sphingosine backbone), and C shows the distribution of the N-acyl chains (expressed as carbon-chain-length:number of double bonds present) for each backbone subtype as a percentage or the total for that backbone. The results for A and C were from RAW264.7 cells placed in culture for 24 h and then analyzed, as done by the LIPID MAPS studies ( in B, the results are for cells in the medium for 4 days (the N-acyl-chain distribution was similar for cells in culture for 4 days (not shown)). The error bars represent the mean ± SD for analysis of four individual dishes in each group.
Biophysical Journal  , DOI: ( /j.bpj )
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4. Figure 2
DSC thermograms of pure Cers. Chain lengths are indicated above the figure. Results (third scans) of two very similar measurements are represented. The arrow indicates 500 Cal/mol × °C. The amount of sample may not be the same in all three cases.
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5. Figure 3
Compression isotherms of pure Cer monolayers. (A–C) Surface pressure versus molecular area of representative isotherms for pure Cers. (D–F) Dependence of compressibility modulus on molecular area.
Biophysical Journal  , DOI: ( /j.bpj )
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6. Figure 4
DSC thermograms of pure C16 SM and binary mixtures with either 10% or 30% of different C16 deoxyceramides. Arrow, 1 kcal/mol × °C. Results (third scans) of two very similar measurements are represented. Thermograms were fitted to the smallest number of symmetrical components.
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7. Figure 5
Compression isotherms of pure C16:0 SM and C16:0 SM-C16:0 Cer mixed monolayers. (A and B) Surface pressure versus molecular area of representative isotherms for pure Cers. (D and E) Dependence of compressibility modulus on molecular area.
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8. Figure 6
Confocal microscopy of Rho-PE-stained pure C16:0 SM vesicles in the presence of either 10% or 30% of different Cers. (A) C16:0 Cer. (B) C16:0 1-deoxyCer. (C) C16:0 1-deoxyDHCer. (D) C16:0 1-deoxymethylDHCer. To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
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9. Figure 7
DSC thermograms of pure C24:1 SM and binary mixtures with either 10% or 30% of different C24:1 deoxyceramides. Arrow, 500 Cal/mol × °C. Results (third scans) of two very similar measurements are represented. Thermograms were fitted to the smallest number of symmetrical components.
Biophysical Journal  , DOI: ( /j.bpj )
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10. Figure 8
Compression isotherms of pure C24:1 SM and C24:1 SM-C24:1 Cer mixed monolayers. (A and B) Surface pressure versus molecular area of representative isotherms for pure Cers. (D and E) Dependence of compressibility modulus (κ) on molecular area.
Biophysical Journal  , DOI: ( /j.bpj )
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11. Figure 9
Confocal microscopy of Rho-PE-stained pure C24:1 SM vesicles in the presence of either 10% (A and C or 30% (B and D) of different Cers. (A) C24:1 Cer. (B) C24:1 1-deoxyCer. (C) C24:1 1-deoxyDHCer. (D) C24:1 1-deoxymethylDHCer. To see this figure in color, go online.
Biophysical Journal  , DOI: ( /j.bpj )
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