1. Volume 7, Issue 12, Pages 943-955 (December 2000)
Cloning, sequencing and analysis of the enterocin biosynthesis gene cluster from the marine isolate ‘Streptomyces maritimus’: evidence for the derailment of an aromatic polyketide synthase 
Jörn Piel, Christian Hertweck, Paul R Shipley, Deanna M Hunt, Mark S Newman, Bradley S Moore 
Chemistry & Biology 
Volume 7, Issue 12, Pages (December 2000)
DOI: /S (00)

2. Figure 1
Proposed biosynthetic pathway from benzoyl-CoA to enterocin and the wailupemycins.
Chemistry & Biology 2000 7, DOI: ( /S (00) )

3. Figure 2
Organization of the enterocin biosynthetic gene cluster (enc) in ‘S. maritimus’. Each arrow represents the direction of transcription of an ORF.
Chemistry & Biology 2000 7, DOI: ( /S (00) )

4. Figure 3
Possible biosynthetic pathways leading to benzoic acid in ‘S. maritimus’. Route A, β-oxidative degradation; route B, retro-aldol degradation with a possible additional chain extension performed by the thiolase EncJ.
Chemistry & Biology 2000 7, DOI: ( /S (00) )

5. Figure 4
Phylogenetic tree of EncH, EncN and related acyl- and aryl-CoA ligases. Sequences were retrieved from GenBank (accession numbers given in parentheses) and aligned with ClustalX. The scale bar indicates 0.1 changes per nt.
Chemistry & Biology 2000 7, DOI: ( /S (00) )

6. Figure 5
Alignment of homologues to EncM around the conserved FAD-binding histidine. Sequence identification codes are Smari_EncM, ‘S. maritimus’ EncM; Bsubt_YgaK, Bacillus subtilis hypothetical protein YgaK (accession number CAB04812); Bsubt_YvdP, B. subtilis hypothetical protein YvdP (CAB08045); Mtu_Rv1736, Mycobacterium tuberculosum hypothetical protein Rv1736 (CAB03710); Slave_McmM, Streptomyces lavendulae McmM (AAD32736); Slave_MitR, S. lavendulae MitR (AAD28454); Aox_6HDNO, A. oxidans 6-hydroxy-D-nicotine oxidase (448109); Ath_F19I3, Arabidopsis thaliana putative berberine bridge enzyme (AAD25758); Speuc_DnrW, Streptomyces peucetius DnrW (AAD00354).
Chemistry & Biology 2000 7, DOI: ( /S (00) )

7. Figure 6
LC-APCI-MS traces of engineered polyketides from (1) S. lividans K4-114 (pSEK4), (2) S. lividans K4-114 (pSEK4/pCH20), and (3) S. lividans K4-114 (pRM5). HPLC was monitored at 254 nm. Chromatographic peaks labeled with letters ‘a’ (MH+=301) and ‘b’ (MH+=285) are uncharacterized polyketides.
Chemistry & Biology 2000 7, DOI: ( /S (00) )

8. Figure 7
(A) SDS–polyacrylamide gel showing the MBP-EncR purified protein preparation. Lane 1: molecular weight markers; lane 2: cell-free preparation; lane 3: MBP-EncR (calculated molecular weight is 86.6 kDa). (B) HPLC traces of standards 5-deoxyenterocin (chromatogram 1) and enterocin (chromatogram 2) and the MBP-EncR catalyzed reaction mixture (chromatogram 3).
Chemistry & Biology 2000 7, DOI: ( /S (00) )