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Alexandre Guilherme Zabeu Rossi, Ph. D

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1 Effects of metoclopramide-induced hyperprolactinemia on the prolactin receptor of murine endometrium 
Alexandre Guilherme Zabeu Rossi, Ph.D., Regina Célia Teixeira Gomes, Ph.D., Manuel de Jesus Simões, Ph.D., Ricardo dos Santos Simões, M.D., Patrícia B. Oliveira, B.S., José Maria Soares, Ph.D., Edmund C. Baracat, Ph.D.  Fertility and Sterility  Volume 93, Issue 5, Pages (March 2010) DOI: /j.fertnstert Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

2 Figure 1 Result of agarose gel electrophoresis of RT-PCR products stained with SYBR Gold. (A) Detection of long (Prl-R-L) mRNA expression in the uteri from all of the groups; (B) detection of short 1 (Prl-R-S1) mRNA expression in the uteri from all of the groups; (C) detection of β-actin (internal control) mRNA expression in the uteri from all of the groups. GI—nonoophorectomized mice given vehicle; GII—nonoophorectomized mice treated with metoclopramide; GIII—oophorectomized mice treated with metoclopramide; GIV—oophorectomized mice treated with metoclopramide and 17β-estradiol; GV—oophorectomized mice treated with metoclopramide and micronized progesterone; GVI—oophorectomized mice treated with metoclopramide and a solution of 17β-estradiol and micronized progesterone. The gels were subjected to 300 nm transillumination. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions

3 Figure 2 Graphic representation of the amount of cDNA of PRL-R (long and short 1 isoforms) and β-actin. cDNA after 32 RT-PCR cycles in the groups. (A) Ratio of long isoform (Prl-R-L)/β-actin; (B) ratio of short 1 isoform (Prl-R-S1)/β-actin; (C) β-Actin values. GI—nonoophorectomized mice given vehicle; GII—nonoophorectomized mice treated with metoclopramide; GIII—oophorectomized mice treated with metoclopramide; GIV—oophorectomized mice treated with metoclopramide and 17β-estradiol; GV—oophorectomized mice treated with metoclopramide and micronized progesterone; GVI—oophorectomized mice treated with metoclopramide and a solution of 17β-estradiol and micronized progesterone. ∗P<.01 compared with GI. Fertility and Sterility  , DOI: ( /j.fertnstert ) Copyright © 2010 American Society for Reproductive Medicine Terms and Conditions


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