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Overview of the analytical workflow used in this study and a representative MS/MS spectrum.a, Overview of the analytical workflow used in this study. Overview.

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Presentation on theme: "Overview of the analytical workflow used in this study and a representative MS/MS spectrum.a, Overview of the analytical workflow used in this study. Overview."— Presentation transcript:

1 Overview of the analytical workflow used in this study and a representative MS/MS spectrum.a, Overview of the analytical workflow used in this study. Overview of the analytical workflow used in this study and a representative MS/MS spectrum.a, Overview of the analytical workflow used in this study. Trypsin digestion of the whole cell lysate was followed by enrichment of phosphopeptides using two stages of chromatography (SCX and TiO2). Phosphopeptides were separated on nano-HPLC and mass-measured and fragmented in the high performance LTQ-Orbitrap mass spectrometer. b, MS/MS spectrum of the phosphopeptide HRDLLGApTNPANALAGTLR from the nucleoside diphosphate kinase (ndk). Precursor (peptide) ion mass was measured in the orbitrap mass analyzer with mass deviation of 0.09 ppm. Rich backbone fragmentation in the MS/MS spectrum localized the phosphorylation site to Thr93. Boris Macek et al. Mol Cell Proteomics 2008;7: © 2008 The American Society for Biochemistry and Molecular Biology

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