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Supplemental Digital Content 3 – Figure 2
Supplemental Digital Content 3 – Figure 2. Gating strategy for identification of activated T cells. Showing one representative viremic individual. Identification of singlets using forward scatter area (FSC-A) versus height (FCS-H) plot (A). Subsequently, lymphocytes were identified through a side scatter area (SSC-A) versus FSC-A plot (B), followed by identification of CD3+CD4+ events (C). Finally HLA-DR+CD38+ (D and F) and Ki67+ (E and G) events were identified within the CD3+CD4+ (D and E) or CD3+CD4- (F and G) populations. For details about staining procedures and antibodies, see methods section. A B C D E F G
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