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Example of MS/MS spectrum of peptide FPTLTGFNR (hypothetical protein with signal peptide EAK88888; N77) from a protein digestion mixture prepared by labeling two separate digests with tag 114 and 115, respectively, and combining the reaction mixtures in a 1:1 ratio.A, isotopic distribution of a doubly charged precursor [M + 2H]2+, m/z Example of MS/MS spectrum of peptide FPTLTGFNR (hypothetical protein with signal peptide EAK88888; N77) from a protein digestion mixture prepared by labeling two separate digests with tag 114 and 115, respectively, and combining the reaction mixtures in a 1:1 ratio.A, isotopic distribution of a doubly charged precursor [M + 2H]2+, m/z B, low mass region showing the signature ions used for quantification. The peptide was labeled by isobaric tags at the N terminus. William J. Snelling et al. Mol Cell Proteomics 2007;6: © 2007 The American Society for Biochemistry and Molecular Biology
![Example of MS/MS spectrum of peptide FPTLTGFNR (hypothetical protein with signal peptide EAK88888; N77) from a protein digestion mixture prepared by labeling two separate digests with tag 114 and 115, respectively, and combining the reaction mixtures in a 1:1 ratio.A, isotopic distribution of a doubly charged precursor [M + 2H]2+, m/z](http://slideplayer.com./slide/16500288/96/images/1/Example+of+MS%2FMS+spectrum+of+peptide+FPTLTGFNR+%28hypothetical+protein+with+signal+peptide+EAK88888%3B+N77%29+from+a+protein+digestion+mixture+prepared+by+labeling+two+separate+digests+with+tag+114+and+115%2C+respectively%2C+and+combining+the+reaction+mixtures+in+a+1%3A1+ratio.A%2C+isotopic+distribution+of+a+doubly+charged+precursor+%5BM+%2B+2H%5D2%2B%2C+m%2Fz.jpg "Example of MS/MS spectrum of peptide FPTLTGFNR (hypothetical protein with signal peptide EAK88888; N77) from a protein digestion mixture prepared by labeling two separate digests with tag 114 and 115, respectively, and combining the reaction mixtures in a 1:1 ratio.A, isotopic distribution of a doubly charged precursor [M + 2H]2+, m/z B, low mass region showing the signature ions used for quantification. The peptide was labeled by isobaric tags at the N terminus. William J. Snelling et al. Mol Cell Proteomics 2007;6: © 2007 The American Society for Biochemistry and Molecular Biology.")
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