1. Increases in phosphorylation of SAPK/JNK and p38MAPK correlate negatively with mouse embryo development after culture in different media 
Yingchun Wang, M.D., Ph.D., Elizabeth E. Puscheck, M.D., M.S., Jennifer J. Lewis, M.S., Anna B. Trostinskaia, M.D., Ph.D., Fangfei Wang, M.D., Daniel A. Rappolee, Ph.D. 
Fertility and Sterility 
Volume 83, Issue 4, Pages (April 2005)
DOI: /j.fertnstert
Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

2. FIGURE 1
p38MAPK phosphorylation is detected more intensely in preimplantation embryos (E3.5) cultured for 24 hours in Ham's F10+BSA (A) than in QCM (B). In B, the bar indicates 100 μM.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

3. FIGURE 2
Stress-activated protein kinase/Jun kinase (SAPK/JNK) phosphorylation is detected more intensely in preimplantation embryos (E3.5) cultured for 24 hours in M-16 (A) than in QCM (B). In B, the bar indicates 100 μM.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

4. FIGURE 3
Correlation of E1.5 embryo preimplantation development during 72-hour culture in different media with the SAPK/JNK phosphorylation fluorescent intensity level. A, 4-cell/compaction rate (Pearson correlation, r = −0.791, P<.05); B, morula formation rate (r = −0.855, P<.05); C, blastocyst formation rate (r = −0.899, P<.01); D, hatching rate (r = −0.808, P<.05).
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions

5. FIGURE 4
Western blot analysis of E3.5 embryo ex vivo, cultured for 24 hours in Ham's F10+BSA and KSOM+AA. Mouse embryo isolated on E3.5 tested for p38MAPK all forms and phosphorylation, SAPK/JNK all forms, and phosphorylation expression by Western blot analysis (described in the Materials and Methods section) showed correct sized bands at 43 kDa for p38MAPK and 46 and 54 kDa for SAPK/JNK. Both SAPK/JNK and p38MAPK phosphorylation was greatly increased when embryos were cultured for 24 hours in Ham's F10, compared with KSOM+AA. Ham's F10 vs. KSOM+AA: SAPK/JNK phospho/SAPK/JNK all forms = 1.45, 0.78; p38MAPK phospho/p38MAPK all forms = 1.15, Note that 80–110 embryos were used in all groups, but some loss of embryo lysate may occur (for example, Ham's F10+BSA group for p38MAPK). Histograms represent SAPK phosphorylation or p38MAPK phosphorylation normalized to all forms.
Fertility and Sterility  , DOI: ( /j.fertnstert )
Copyright © 2005 American Society for Reproductive Medicine Terms and Conditions