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Quantitative Amplification of Genomic DNA from Histological Tissue Sections after Staining with Nuclear Dyes and Laser Capture Microdissection  Torsten.

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Presentation on theme: "Quantitative Amplification of Genomic DNA from Histological Tissue Sections after Staining with Nuclear Dyes and Laser Capture Microdissection  Torsten."— Presentation transcript:

1 Quantitative Amplification of Genomic DNA from Histological Tissue Sections after Staining with Nuclear Dyes and Laser Capture Microdissection  Torsten Ehrig, Sarki A. Abdulkadir, Suzanne M. Dintzis, Jeffrey Milbrandt, Mark A. Watson  The Journal of Molecular Diagnostics  Volume 3, Issue 1, Pages (February 2001) DOI: /S (10) Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 DNA recoveries evaluated by TaqMan PCR. Staining procedures: NS, no stain; TB, toluidine blue O; AB, azure B; MG, methyl green; H, Mayer's hematoxylin. Tissue was sampled by manual gross dissection from histological slides (A and B) or by LCM (C and D). The original tissue samples were formalin-fixed, paraffin-embedded archival surgical pathology tissue (A and C) or unfixed, frozen surgical tumor material (B and D). The y-axis gives the amount of template DNA as determined by TaqMan PCR of genomic β-actin DNA. The indicated amount of DNA refers either to a sampled histological tissue section approximately 1 cm2in area (A and B) or to tissue sampled by 500 LCM firings (C and D). Each data point is the average from two independently processed histological slides. The difference between NS and TB, AB, or MG in B is statistically significant (P < 0.05). The Journal of Molecular Diagnostics 2001 3, 22-25DOI: ( /S (10) ) Copyright © 2001 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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