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Plot of the deviation of the predicted pI value of every peptide spectrum from the average pI calculated for each fraction for validated (a) and non-validated spectra (b) against the peptide score. Plot of the deviation of the predicted pI value of every peptide spectrum from the average pI calculated for each fraction for validated (a) and non-validated spectra (b) against the peptide score. The inset visualizes the percentage of spectra that have deviations within the size of the pH segment covered by one well (i.e. 0 ± 0.13 pH units), by two wells (0 ± 0.26), by three wells (0 ± 0.39), etc. Patric Hörth et al. Mol Cell Proteomics 2006;5: © 2006 The American Society for Biochemistry and Molecular Biology
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