1. Gel Electrophoresis

2. Scenario:
Mr. I.M. Megabucks, the wealthiest man in the world, recently died. Since his death, three women have come forward. Each woman claims to have a child by Megabucks and demands a substantial share of his estate for her child.
How can we find out who is telling the truth??

3. The birth of gel electrophoresis
Gel Electrophoresis: A laboratory method used to separate mixtures of DNA according to molecular size. 
What is it?
The birth of gel electrophoresis

4. A.k.a. DNA Fingerprinting
DNA is extracted from a cell (Blood, saliva, etc)
Restriction enzymes are used to recognize & cut at specific sequences in the DNA
Cut up DNA fragments are loaded into wells on an agarose gel square

5. Which would you rather drive if you wanted to move through traffic the quickest??B D C

6. 4. Electrodes are attached and an electric current moves negatively charged DNA fragments toward the + pole 5. Large fragments move slower than small fragments, creating a distinct banding pattern 6. Bands are dyed &compared for similarities/ Differences under UV light

8. Man set free after 17 years in prison

10. Practical Applications
Taxonomists use it help distinguish species
Evolutionary Biologists can compare similarities and create family trees
Crime Scene Analysis
Paternity testing