1. Aberrant expression of filaggrin in SASP−/− hairless mice.
Aberrant expression of filaggrin in SASP−/− hairless mice. A. Immunofluorescence staining of frozen sections of the back skin of SASP+/− and SASP−/− mice stained with anti‐filaggrin antibody (red). Nuclei were counterstained with bisbenzimide (blue). The SASP−/− epidermis showed an increased amount of filaggrin‐positive stained lower SC. Dashed lines represent the border between the epidermis and dermis. BF, bright field. Scale bar: 10 µm. B. Enlarged view of A shows an increased amount of lower SC layers in the SASP−/− hairless epidermis. C. Equivalent amounts of tape‐stripped extracts (10 times, 5 µg) from SASP+/+ (n = 2), SASP+/− (n = 2), and SASP−/− (n = 2) mice were immunoblotted with anti‐filaggrin antibodies. CBB staining of extracts revealed a reduced expression of filaggrin monomer bands and other major SC proteins in the SASP−/− hairless mice epidermis (left; Coomassie). Immunoblotting with anti‐filaggrin demonstrated that an accumulation of aberrant filaggrin degradation products (dimer and trimer sized) was detected (right), whereas mature filaggrin was rarely detected. As equivalent amounts of SC extracts were loaded, the intensity of the profilaggrin band was decreased in SASP−/−, possibly due to an increase in the concentrations of other smear proteins.
Takeshi Matsui et al. EMBO Mol Med. 2011;3:
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