1. MODELING TEAM
REPORT
July 28, 2009
Mary & Leon

2. CONTENTS 1 Modeling Plan for SiGEM 2
Further Analysis on the IDO Enzyme Model 2
Mutated and Regular Trp-Repressor 3
The Production of B-carotene with IPP 4
BLH Enzyme Kinetics 5

3. 1.
Modeling Plan
for SiGEM

4. Modeling Team Subprojects
BLH
Ming
Trp-R
Mark
Anusuya
NO-SoxS
Suzie
Crt
Bobby
IL-6 Export
Robert
IDO
Ariana
Joe

5. 2.
Further Analysis on
the IDO Enzyme Model

6. The Reaction: 5MT--|Trp
REACTION 1：L-tryptophan + O2 = N-formyl-L-kynurenine E
REACTION 2: 5-methyl-tryptophan + O2
=4-(2-amino-5-methylphenyl)-2-(formylamino)-4-oxobutanoic acid

7. Observations

8. Observations

9. Observations

10. An Explanation with Equations

11. Speeding Up the Reaction
[S]0 = 10mM
[S]0 = 2.28e-6mM

12. 3.
Mutated and Regular
Trp-Repressor

13. The Reaction: Trp/5MTDNA-P/O
Input
Output

14. Equations

15. Observation: [Trp]=0~0.01M

16. Observation: [Trp]=0~0.001M

17. The Reaction: 5MTIL-6
Output
Input

18. Observation: 5MT->IL-6
Level of il-6 over time
IL-6 Export?

19. 4.
The Production of
B-carotene with IPP

20. The Reaction: 8*IPP->B-Carotene
Output
Input

21. 5.
BLH Enzyme
Kinetics

22. The Reaction: B-CaroteneRetinoic Acid
Output
Input

23. Observations
B-carotene input?
BLH levels?

24. Thanks for your attention^^