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Outbreak of hand, foot and mouth disease/herpangina associated with coxsackievirus A6 and A10 infections in 2010, France: a large citywide, prospective.

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Presentation on theme: "Outbreak of hand, foot and mouth disease/herpangina associated with coxsackievirus A6 and A10 infections in 2010, France: a large citywide, prospective."— Presentation transcript:

1 Outbreak of hand, foot and mouth disease/herpangina associated with coxsackievirus A6 and A10 infections in 2010, France: a large citywide, prospective observational study  A. Mirand, C. Henquell, C. Archimbaud, S. Ughetto, D. Antona, J.-L. Bailly, H. Peigue-Lafeuille  Clinical Microbiology and Infection  Volume 18, Issue 5, Pages E110-E118 (May 2012) DOI: /j x Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

2 FIG. 1 Distribution of the number of enterovirus-positive specimens and prevalent serotypes by month between April and December CV-A, coxsackievirus A; EV, enterovirus; HEVB, human enterovirus B species. Clinical Microbiology and Infection  , E110-E118DOI: ( /j x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

3 FIG. 2 Methodological approach and results of enterovirus (EV) genotyping. Prospective EV genotyping was attempted in the 143 consecutive patients with EV-positive specimens with the same extract used for the EV genome detection assay. Identification was performed from the complete 1D sequence for 107 (74.8%) specimens and from partial 1A–1B sequence for 22 of the 36 remaining samples. For 12 patients, EV identification was obtained with the strains isolated in cell culture by genotyping of the complete 1D sequence (n = 10) or partial 1A–1B sequence (n = 2). CV, coxsackievirus; HEVB, human enterovirus B species. Clinical Microbiology and Infection  , E110-E118DOI: ( /j x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions

4 FIG. 3 Phylogenetic trees based on partial VP1 coding sequence of (a) coxsackievirus A10 (246 nucleotides) and (b) coxsackievirus A6 (293 nucleotides). Genetic distances were calculated with the Tamura–Nei model of evolution. The tree was constructed by the neighbour-joining method and validated with 1000 pseudo-replicates. Only bootstrap values of over 70% are shown. Branch length was drawn to the indicated scale (proportion of nucleotide per site). Taxon names have been deleted for clarity (see Supplementary material; Fig. S1). Sequences of strains isolated in Finland are indicated in blue, in France in red, and in other European countries in green. Clinical Microbiology and Infection  , E110-E118DOI: ( /j x) Copyright © 2012 European Society of Clinical Infectious Diseases Terms and Conditions


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