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Coronary Microvascular Reactivity After Ischemic Cold Storage and Reperfusion  Charles O Murphy, MD, Pan- Chih, MD, John Parker Gott, MD, Robert A Guyton,

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Presentation on theme: "Coronary Microvascular Reactivity After Ischemic Cold Storage and Reperfusion  Charles O Murphy, MD, Pan- Chih, MD, John Parker Gott, MD, Robert A Guyton,"— Presentation transcript:

1 Coronary Microvascular Reactivity After Ischemic Cold Storage and Reperfusion 
Charles O Murphy, MD, Pan- Chih, MD, John Parker Gott, MD, Robert A Guyton, MD  The Annals of Thoracic Surgery  Volume 63, Issue 1, Pages (January 1997) DOI: /S (96)

2 Fig. 1 Responses to acetylcholine in vitro of canine coronary microvessels from the right ventricle in control hearts (CON; n = 10) and in hearts after 3 hours of cold ischemic storage using crystalloid cardioplegia (CCP; n = 8), Roe’s solution (ROE; n = 8), or University of Wisconsin solution (UW; n = 8) followed by 1 hour of reperfusion. Microvessels were pressurized (20 mm Hg) in a no-flow state and preconstricted with endothelin I by 30% to 40% of the baseline diameter. (SEM = standard error of the mean; ∗p < 0.05 compared with control.) The Annals of Thoracic Surgery  , 20-27DOI: ( /S (96) )

3 Fig. 2 Maximum response to calcium ionophore at a maximum concentration of 3 × 10−5 mol/L in vitro of canine coronary microvessels from the right ventricle in control hearts (CON; n = 10) and in hearts after 3 hours of cold ischemic storage using crystalloid cardioplegia (CCP; n = 8), Roe’s solution (ROE; n = 8), or University of Wisconsin solution (UW; n = 8) followed by 1 hour of reperfusion. (∗p < 0.05 compared with control.) The Annals of Thoracic Surgery  , 20-27DOI: ( /S (96) )

4 Fig. 3 Maximum response to acetylcholine at a maximum concentration of 3 × 10−5 mol/L in vitro of canine coronary microvessels from the left ventricle in control hearts (CON; n = 10) and in hearts after 3 hours of cold ischemic storage using crystalloid cardioplegia (CCP; n = 8), Roe’s solution (ROE; n = 8), or University of Wisconsin solution (UW; n = 8) followed by 1 hour of reperfusion. (∗p < 0.05 compared with control.) The Annals of Thoracic Surgery  , 20-27DOI: ( /S (96) )

5 Fig. 4 Maximum response to calcium ionophore at a maximum concentration of 3 × 10−5 mol/L in vitro of canine coronary microvessels from the left ventricle in control hearts (CON; n = 10) and in hearts after 3 hours of cold ischemic storage using crystalloid cardioplegia (CCP; n = 8), Roe’s solution (ROE; n = 8), or University of Wisconsin solution (UW; n = 8) followed by 1 hour of reperfusion. (∗p < 0.05 compared with control.) The Annals of Thoracic Surgery  , 20-27DOI: ( /S (96) )

6 Fig. 5 Maximum response to acetylcholine at a maximum concentration of 3 × 10−5 mol/L in vitro of canine coronary microvessels from the right ventricle (RV) in all groups (n = 24) and the left ventricle (LV) in all groups (n = 24) after 3 hours of cold ischemic storage followed by 1 hour of reperfusion. (∗p < 0.05 compared with combined left ventricular groups.) The Annals of Thoracic Surgery  , 20-27DOI: ( /S (96) )

7 Fig. 6 Maximum response to calcium ionophore at a maximum concentration of 3 × 10−5 mol/L in vitro of canine coronary microvessels from the right ventricle (RV) in all groups (n = 24) and the left ventricle (LV) in all groups (n = 24) after 3 hours of cold ischemic storage followed by 1 hour of reperfusion. (∗p < 0.05 compared with combined left ventricular groups.) The Annals of Thoracic Surgery  , 20-27DOI: ( /S (96) )


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