1. Table 2. Set of reference peptide for retention time correction
Peptide Sequence
HF* HF
SSAAPPPPPR
7.6
MGVPYAIIK
30.6
GISNEGQNASIK
15.5
GILFVGSGVSGGEEGAR
34.5
HVLTSIGEK
15.6
SFANQPLEVVYSK
35.0
DIPVPKPK
17.7
IADISLAAFGR
35.8
NAGGVGELEK
18.3
LTILEELR
37.3
IGDYAGIK
19.2
EYVPSVIEPSFGIGR
40.1
GISSSAIPYSR
23.2
NGFILDGFPR
40.4
DLETLTMHTK
23.5
ELASGLSFPVGFK
41.2
YYTITEVATR
25.4
LSSEAPALFQFDLK
46.7
ELGQSGVDTYLQTK
28.4
*HF refers to the hydrophobicity factor calculated using the SSRCalc algorithm [1, 2].
[1] Krokhin, O. V., Sequence-specific retention calculator. Algorithm for peptide retention prediction in
ion-pair RP-HPLC: application to 300- and 100-A pore size C18 sorbents. Anal Chem (2006).
[2] Spicer, V., Yamchuk, A., Cortens, J., Sousa, S., et al., Sequence-specific retention calculator. A family of peptide retention time prediction algorithms in reversed-phase HPLC: applicability to various
chromatographic conditions and columns. Anal Chem (2007).