1. Effect of NTRs on NPCs.
Effect of NTRs on NPCs. (A–D) Images from a video sequence (see Video 1) of the cytoplasmic side of the NE, in which, step-by-step, many of the proteins (and chemical energy) required for the classical import cycle of NLS proteins are added to the system. (A) Cytoplasmic side of NE. (B) After addition of the Ran mix and energy mix. (C)hsNTF2 (0.7 μM) is added to the sample. (D)hsImpβ (1 μM) is added and all NPCs fill with protein. (E–H) The rotationally averaged height profiles of the cross-correlation averaged NPCs from the images displayed in (A–D), respectively, showing a filling of the pore lumen and some increase in the pore rim height upon incubation with hsImpβ. (I) Nucleoplasmic side of the membrane before (top) and after (bottom) addition of hsImpβ (1 μM). (J, K) Cropped pores from the image sequence (A–D) showing changes as a function of time. Scale bars: 600 nm (A–D), 300 nm (I), and 100 nm (J and K). Colour scales: 150 nm (A–D) and 80 nm (I–K).
George J Stanley et al. LSA 2018;1:e
© 2018 Stanley et al.