1. Comparison of the original ITS primer pair and the new, modified ITS primer pair.
Comparison of the original ITS primer pair and the new, modified ITS primer pair. (A) Procrustes plot of original and modified ITS1 constructs, Bray-Curtis dissimilarity M2 = (B) Pie charts illustrating the mean relative abundance of phyla present (all studies combined) in samples amplified with the old ITS construct and the modified ITS construct. (C) Taxa scatterplots for the original and modified ITS primers. Phyla plots are shown for American Gut fecal, American Gut skin, agricultural soils, EMP Rice Rhizome, Body Farm 1, Body Farm 2, mouse decomposition, and Sloan built environment samples. Outlier samples have been removed from the data shown.
William Walters et al. mSystems 2016; doi: /mSystems