1. Diagram of experimental design.
Diagram of experimental design. Bulk feces were collected from humans and dogs, homogenized, treated with a range of common preservatives, and subjected to a range of likely temperature scenarios. Conditions not sampled are marked with a gray X. Additional specific combinations of conditions not sampled are described in Materials and Methods and listed in Table S2 in the supplemental material. For freshly collected samples in no preservative, six replicate subsamples were taken from the same primary aliquot. At the 8-week time point, six replicate subsamples were taken from samples with no preservative and samples stored in 95% ethanol. rmtp, room temperature.
Se Jin Song et al. mSystems 2016; doi: /mSystems