1. Vitrification is not superior to rapid freezing of normozoospermic spermatozoa: effects on sperm parameters, DNA fragmentation and hyaluronan binding 
Azam Agha-Rahimi, Mohammad Ali Khalili, Ali Nabi, Sareh Ashourzadeh 
Reproductive BioMedicine Online 
Volume 28, Issue 3, Pages (March 2014)
DOI: /j.rbmo
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2. Figure 1
Comparison of sperm parameters after 24h for vitrification and rapid freezing methods. All parameters were significantly lower for rapid freezing and vitrification compared with fresh samples (P<0.0001). Values are mean and standard deviation.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
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3. Figure 2
(A) Classification of abnormal morphology in fresh, rapid freezing and vitrification groups; sperm tail and midpiece defects increased after cryopreservation (P<0.0001); values are mean and standard deviation. (B) Photomicrograph of frozen–thawed spermatozoa showing tail abnormalities including coiled (thin arrow) and bent (bold arrow); bar=10μm.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions

4. Figure 3
Pearson correlation between total motility and viability (A) and morphology (B) in vitrification group.
Reproductive BioMedicine Online  , DOI: ( /j.rbmo )
Copyright © 2013 Reproductive Healthcare Ltd. Terms and Conditions