1. Extraction of proteins from MALDI IMS slides.
Extraction of proteins from MALDI IMS slides.A, the sinapinic acid matrix layer of a ready-to-measure MALDI IMS microscopy slide (here prepared from normal stomach tissue is removed by a two-step extraction procedure using 7.5 and 60% acetonitrile in 0.2% tri-fluoro acetic acid (TFA). The remaining tissue is recovered by means of a scalpel (see also supplemental Fig. S1A) B, silver stained SDS-PAGE of the proteins recovered from the microscopy slide showing that the acetonitrile extractions primarily recover low molecular weight proteins (see also supplemental Fig. S1B).
Stefan K. Maier et al. Mol Cell Proteomics 2013;12:
© 2013 by The American Society for Biochemistry and Molecular Biology, Inc.