1. Molecular Biotechnology *
1-HindIII
2-EcoRI
3 –BamHI
4 – PstI
5- Molecular weight marker
* hybridization signal (weak, more clear in the membrane)
Since there is no specific hybridization signal in all the wells of this membrane, use for your report the “reference membrane “ for the comparison with the in silico analysis.
Do not forget to compare this result with the gel of the restriction of genomic DNA – try to explain why there is no signal in the other lanes