1. Functional RNAs expand the synthetic biological toolbox
Isaacs et al., Nat. Biotechnol. 2006

2. Engineered translation regulators
Isaacs et al., Nat. Biotechnol. 2004

3. Translation riboregulator pairs are specific making the technique scalable
Isaacs et al., Nat. Biotechnol. 2004

4. Allosteric riboregulators of gene expression
Base-pairing specificity = scalability
Bayer and Smolke., Nat. Biotechnol. 2005

5. Antiswitches are modular and tunable
Bayer and Smolke., Nat. Biotechnol. 2005

6. Riboswitches – Natural Translational Control
Tabor et al., Biotech and Gen Eng. Rev, 2006

7. Riboswitches can regulate via Boolean logic
Sudarsan et al., Science 2006

8. Regulation of protein decay – postranslational control
-ssrA proteolysis system
Tag sequence:
NH3-Protein-AANDENYALAA-COOH
Andersen et al., Appl. Environ. Micro., 1998

9. Standard Assembly (Combining biobricks)

10. Build a synthetic gene expression system with XOR logic
Mayo et al., PLoS Biology, 2006