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Identification of drug- and drug-metabolite immune responses originating from both naive and memory T cells  Andrew Gibson, MRes, Lee Faulkner, PhD, Sally.

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Presentation on theme: "Identification of drug- and drug-metabolite immune responses originating from both naive and memory T cells  Andrew Gibson, MRes, Lee Faulkner, PhD, Sally."— Presentation transcript:

1 Identification of drug- and drug-metabolite immune responses originating from both naive and memory T cells  Andrew Gibson, MRes, Lee Faulkner, PhD, Sally Wood, BSc, B. Kevin Park, PhD, Dean J. Naisbitt, PhD  Journal of Allergy and Clinical Immunology  Volume 140, Issue 2, Pages e5 (August 2017) DOI: /j.jaci Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

2 Fig 1 Naive and memory T cells were exposed to SMX-NO and SMX for 8 days and then recall responses tested. Proliferative responses to SMX-NO (A) and SMX (B) were measured by [3H]-thymidine incorporation (n = 3). Error bars indicate SD of triplicate wells (*P ≤ .05, **P ≤ .005, and ***P < .001). IL-13 secretion to SMX-NO (C) and SMX (D) was measured by ELISpot (n = 3). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

3 Fig 2 T-cell clones were isolated from 2 donors following exposure of naive and memory T cells to SMX-NO and SMX. A, Generation and phenotype of T-cell clones. B and C, Proliferation (SMX, 2 from naive and 5 from memory precursors; SMX-NO, 5 from naive and 2 from memory precursors) and (D and E) secretion of cytokines/cytolytic molecules by T-cell clones isolated following SMX and SMX-NO exposure. Error bars indicate SD of triplicate wells (*P ≤ .05, **P ≤ .005, and ***P < .001). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

4 Fig E1 Overview of study design and results. Delineation of naive and memory T-cell responses to SMX-derived antigens in drug-naive donors. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

5 Fig E2 Comparative ability of DCs and B cells to induce naive and memory T-cell responses to drug-antigen in vitro. Naive and memory T cells from drug-naive donors were exposed to SMX-NO and SMX for 8 days in the presence of EBV-transformed B cells (A) or DCs (B), and then recall responses tested by [3H]-thymidine incorporation. Error bars indicate SD of triplicate wells. Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

6 Fig E3 Antigen cross-reactivity of T-cell clones derived from priming in drug-naive donors and hypersensitive patients. Naive and memory T cells from drug-naive donors were exposed to SMX and SMX-NO for 8 days and then recall responses tested. Cross-reactivity to SMX-NO and SMX was measured by [3H]-thymidine incorporation and/or ELISpot after exposure of naive and memory T cells to SMX-NO (A, n = 3) and SMX (B, n = 3), or exposure of representative SMX-NO– (C) and SMX-responsive (D) T-cell clones from hypersensitive patients. Error bars indicate SD of triplicate wells (*P ≤ .05, **P ≤ .005, and ***P < .001). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

7 Fig E4 MHC I and II restriction of SMX-responsive T-cell clones derived from drug-naive donors. Naive or memory-derived SMX-responsive T-cell clones from drug-naive donors were cultured with autologous EBV-transformed B cells and SMX, with or without MHC I– or II–blocking antibodies for 48 hours. Proliferative responses were measured by [3H]-thymidine incorporation for a further 16 hours. Error bars indicate SD of triplicate wells (*P ≤ .05, **P ≤ .005, and ***P < .001). Journal of Allergy and Clinical Immunology  , e5DOI: ( /j.jaci ) Copyright © 2017 American Academy of Allergy, Asthma & Immunology Terms and Conditions

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