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Suicide Ideation in the depression group (NBB)

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Presentation on theme: "Suicide Ideation in the depression group (NBB)"— Presentation transcript:

1 Suicide Ideation in the depression group (NBB)
A medical term for thoughts about or an unusual preoccupation with suicide. The range varies greatly from fleeting to detailed planning, role playing, self-harm and attempts Anterior Cingulate Cortex * * ** DLPFC was also checked, however, none of the target genes were unchanged. Juan Zhao and Dick Swaab, submitted

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4 Microglia isolation procedure
Mechanic & enzymatic dissociation Cell separation: Percoll gradient Take off myelin Collect cells from interface Stain cells with labeled antibodies: CD11b - PE CD45 - FITC CD15 - APC Selection using magnetic CD11b beads Melief et al. Glia 2012

5 Isolation and phenotyping of pure primary human microglia

6 Peripheral inflammation impacts on microglia activation
Melief et al. Glia 2012

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8 The Netherlands Brain Bank for Psychiatry

9 Phenotype & Characterize human post mortem microglia
Jeroen Melief, thesis 2014

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