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Suicide Ideation in the depression group (NBB)
A medical term for thoughts about or an unusual preoccupation with suicide. The range varies greatly from fleeting to detailed planning, role playing, self-harm and attempts Anterior Cingulate Cortex * * ** DLPFC was also checked, however, none of the target genes were unchanged. Juan Zhao and Dick Swaab, submitted
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Microglia isolation procedure
Mechanic & enzymatic dissociation Cell separation: Percoll gradient Take off myelin Collect cells from interface Stain cells with labeled antibodies: CD11b - PE CD45 - FITC CD15 - APC Selection using magnetic CD11b beads Melief et al. Glia 2012
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Isolation and phenotyping of pure primary human microglia
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Peripheral inflammation impacts on microglia activation
Melief et al. Glia 2012
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The Netherlands Brain Bank for Psychiatry
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Phenotype & Characterize human post mortem microglia
Jeroen Melief, thesis 2014
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