1. TrkB-T1 is upregulated in cerebellum of Pex14ΔC/ΔC BL/ICR mouse at P3.
TrkB-T1 is upregulated in cerebellum of Pex14ΔC/ΔC BL/ICR mouse at P3. (A) Sagittal sections of the cerebellum from wild-type (+/+) and Pex14ΔC/ΔC (ΔC/ΔC) BL/ICR mice were labeled with anti-TrkB (green) and calbindin-D28k (red) antibodies. Scale bar, 20 μm. Higher magnification images of the boxed regions are shown and cell boundaries were indicated as a dashed line (inset). Scale bar, 5 μm. (B) TrkB-positive dots (arrowheads) were quantified (n = 13). (C) Sagittal sections of the cerebellum at P3 were stained with antibodies against vGlut2 (red) and calbindin-D28k (white). The number of dots stained with anti-vGlut2 antibody was quantified (lower panel, n = 4). Scale bar, 10 μm. (D) Sagittal sections of the cerebellum at P3 were stained with antibodies against TrkB (green), vGlut2 (red), and calbindin-D28k (blue). Scale bar, 10 μm. Higher magnification images of the boxed regions are shown (inset). Scale bar, 5 μm. Arrowheads indicate TrkB-positive punctate structures that partly coincided with or are located adjacent to vGlut2-positive punctate structures. (E) Cerebellum lysates were analyzed by SDS-PAGE and immunoblotting with antibodies against TrkB, Pex14pC, and α-tubulin (left panel). TrkB-TK+, full-length TrkB; TrkB-T1, a truncated isoform of TrkB. Amounts of TrkB-TK+ and TrkB-T1 are presented relative to those of TrkB-TK+ in the control mice (middle panel, n = 6). A schematic view of TrkB variants is shown on the right (right panel). TK, tyrosine kinase domain. (F, G) mRNA levels of TrkB-TK+ (F) and TrkB-T1 (G) were quantified by real-time PCR (n = 6). ns, not significant, **P < 0.01, ***P < 0.001, by t test (B, C, E–G). Source data are available for this figure.
Yuichi Abe et al. LSA 2018;1:e
© 2018 Abe et al.