Presentation is loading. Please wait.

Presentation is loading. Please wait.

CRISPR knockdown can be multiplexed to modulate expression of two genes without cross‐talk CRISPR knockdown can be multiplexed to modulate expression of.

Published byPosy Moody Modified over 5 years ago

Similar presentations

Presentation on theme: "CRISPR knockdown can be multiplexed to modulate expression of two genes without cross‐talk CRISPR knockdown can be multiplexed to modulate expression of."— Presentation transcript:

1 CRISPR knockdown can be multiplexed to modulate expression of two genes without cross‐talk
CRISPR knockdown can be multiplexed to modulate expression of two genes without cross‐talk Schematic of the strain expressing two reporters and PTet‐dCas9 integrated in the chromosome at phage attachment sites. The levels of the two reporters can be controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs (squares), and also carrying the tracrRNA sequence (not shown).Relative GFP and RFP concentration given relatively to the non‐targeting spacer measured by high‐throughput microscopy for a collection of 20 CRISPR plasmids. Each point represents a single cell, and each color represents the population obtained with one CRISPR plasmid. The overlaid meshwork connects the median values of the different populations. Source data are available online for this figure. Antoine Vigouroux et al. Mol Syst Biol 2018;14:e7899 © as stated in the article, figure or figure legend

Download ppt "CRISPR knockdown can be multiplexed to modulate expression of two genes without cross‐talk CRISPR knockdown can be multiplexed to modulate expression of."

Similar presentations

Fus3 is required for deletions of GIM4 and BIM1 to increase cell‐to‐cell variability Fus3 is required for deletions of GIM4 and BIM1 to increase cell‐to‐cell.

Fus3 is required for deletions of GIM4 and BIM1 to increase cell‐to‐cell variability Fus3 is required for deletions of GIM4 and BIM1 to increase cell‐to‐cell.
Cluster analysis of 50 genes identified as affecting variability and or pheromone response output Cluster analysis of 50 genes identified as affecting.

Cluster analysis of 50 genes identified as affecting variability and or pheromone response output Cluster analysis of 50 genes identified as affecting.
Microtubule perturbations affect pathway variability η2(P) and transmitted signal P at or upstream of the Ste5 recruitment step Microtubule perturbations.

Microtubule perturbations affect pathway variability η2(P) and transmitted signal P at or upstream of the Ste5 recruitment step Microtubule perturbations.
GFP‐Sed5p localization defect in sgt2Δ.

GFP‐Sed5p localization defect in sgt2Δ.
Statistical colorings of 1H NMR estimates of biochemical variables.

Statistical colorings of 1H NMR estimates of biochemical variables.
CRISPR + CAS = Defensive or Immune System

CRISPR + CAS = Defensive or Immune System
Abundance of proteins matching selected subcellular locations and functions in CaCo‐2 cells. Abundance of proteins matching selected subcellular locations.

Abundance of proteins matching selected subcellular locations and functions in CaCo‐2 cells. Abundance of proteins matching selected subcellular locations.
Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.

Yeast Tgl3p expressed in HeLa cells localizes to lipid droplets.
Schematic diagram of the three circuits analysed in this work.

Schematic diagram of the three circuits analysed in this work.
CRISPR knockdown of the mrdAB operon increases cell width at high repression strengths CRISPR knockdown of the mrdAB operon increases cell width at high.

CRISPR knockdown of the mrdAB operon increases cell width at high repression strengths CRISPR knockdown of the mrdAB operon increases cell width at high.
Statistical analysis of the influence of network regulators on topology clusters in the oleate network. Statistical analysis of the influence of network.

Statistical analysis of the influence of network regulators on topology clusters in the oleate network. Statistical analysis of the influence of network.
Overview of the experimental setting.

Overview of the experimental setting.
Summary of all the reactions considered in the SmartCell simulation of the three circuits. Summary of all the reactions considered in the SmartCell simulation.

Summary of all the reactions considered in the SmartCell simulation of the three circuits. Summary of all the reactions considered in the SmartCell simulation.
Immune features from Model 3 that associate with and predict the HAI response to TIV. The cross‐validated area under the ROC curve (cvAUROC) for Model.

Immune features from Model 3 that associate with and predict the HAI response to TIV. The cross‐validated area under the ROC curve (cvAUROC) for Model.
(A) Graph depicting interaction between the five regulatory motifs measured by synergy and cooccurrence. (A) Graph depicting interaction between the five.

(A) Graph depicting interaction between the five regulatory motifs measured by synergy and cooccurrence. (A) Graph depicting interaction between the five.
Analysis of in silico flux changes along the exponential growth phase: (A) In silico flux changes from 24 to 36 h, from 36 to 48 h, and from 48 to 60 h.

Analysis of in silico flux changes along the exponential growth phase: (A) In silico flux changes from 24 to 36 h, from 36 to 48 h, and from 48 to 60 h.
Epistatic interactions and gene expression variation.

Epistatic interactions and gene expression variation.
In vivo optimization of the tagging approach using the Act5C model locus and flow cytometry-based quantification of the Act5C-GFP tagging success. In vivo.

In vivo optimization of the tagging approach using the Act5C model locus and flow cytometry-based quantification of the Act5C-GFP tagging success. In vivo.
Identification of progenitor states in human cord blood hematopoiesis

Identification of progenitor states in human cord blood hematopoiesis
Experimental design for high‐throughput characterization of synthetic human gut microbiome consortia Experimental design for high‐throughput characterization.

Experimental design for high‐throughput characterization of synthetic human gut microbiome consortia Experimental design for high‐throughput characterization.

Similar presentations

Ads by Google