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CRISPR knockdown can be multiplexed to modulate expression of two genes without cross‐talk CRISPR knockdown can be multiplexed to modulate expression of.

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Presentation on theme: "CRISPR knockdown can be multiplexed to modulate expression of two genes without cross‐talk CRISPR knockdown can be multiplexed to modulate expression of."— Presentation transcript:

1 CRISPR knockdown can be multiplexed to modulate expression of two genes without cross‐talk
CRISPR knockdown can be multiplexed to modulate expression of two genes without cross‐talk Schematic of the strain expressing two reporters and PTet‐dCas9 integrated in the chromosome at phage attachment sites. The levels of the two reporters can be controlled using a plasmid‐borne CRISPR array coding for guide RNAs (diamonds) interspaced with CRISPR repeat motifs (squares), and also carrying the tracrRNA sequence (not shown).Relative GFP and RFP concentration given relatively to the non‐targeting spacer measured by high‐throughput microscopy for a collection of 20 CRISPR plasmids. Each point represents a single cell, and each color represents the population obtained with one CRISPR plasmid. The overlaid meshwork connects the median values of the different populations. Source data are available online for this figure. Antoine Vigouroux et al. Mol Syst Biol 2018;14:e7899 © as stated in the article, figure or figure legend

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