1. Digitized image analysis using CEREB B-Mode Assist

2. Use of CEREB B-Mode Assist System
The standard settings of the ultrasound machine has to be used.
Similar image brightness has to be set for all evaluated images.
The same gain and time gain compensation (TGC) has to be used for evaluation of changes in SN echogenicity in a particular patients during a study.
TCS images has to be save in DICOM or bitmap (jpg, bmp, tiff, png, etc.) formats

3. Loading the TCS image (DICOM or bitmap format)

4. Open the TCS image

5. Use a manual positioning

6. Mark a 5 cm distance on the TCS scale

8. Place the square ROI (5 x 5 cm) in the midbrain area

9. Select the structure specified ROI

10. Place the selected ROI to the SN area

11. Start the SN area analysis

12. Save the counted values

13. Result: echogenicity index=SUM/100

14. Result: echogenicity index=SUM/100
90th percentile of a healthy population (red line)
pathological
normal
Evaluated patient (black line)
Echogenicity index = 18.17

15. The machine specific 90th percentile line (90th percentile of the healthy population SN echogenicity) could be uploaded in each center.