1. Metabolic acidosis inhibits soft tissue calcification in uremic rats
F.J. Mendoza, I. Lopez, A. Montes de Oca, J. Perez, M. Rodriguez, E. Aguilera-Tejero 
Kidney International 
Volume 73, Issue 4, Pages (February 2008)
DOI: /sj.ki
Copyright © 2008 International Society of Nephrology Terms and Conditions

2. Figure 1
Mineral content ((a) calcium, (b) phosphorus) of the aorta in sham-operated rats (Sham) and uremic rats treated with vehicle (5/6 Nx) or with calcitriol 80 ng kg−1 i.p. q.o.d. for 14 days (5/6 Nx+CTR). All groups are shown with normal pH and with acidosis, which was induced by administration of NH4Cl in drinking water at 0.75% (Sham+AC 0.75%), 0.5% (5/6 Nx+AC 0.5% and 5/6 Nx+CTR+AC 0.5%), or 0.3% (5/6 Nx+CTR+AC 0.3%). aP<0.01 vs 5/6 Nx+CTR.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2008 International Society of Nephrology Terms and Conditions

3. Figure 2
Aortic calcification. von Kossa-stained tissue sections of the aorta in sham-operated rats (Sham, a), in uremic rats with normal pH (5/6 Nx, b) and with acidosis (5/6 Nx+AC 0.5%, c), and in uremic rats treated with calcitriol, 80 ng kg−1 i.p. q.o.d. for 14 days with normal pH (5/6 Nx+CTR, d) and with acidosis: 0.5% NH4Cl (5/6 Nx+CTR+AC 0.5%, e) and 0.3% NH4Cl (5/6 Nx+CTR+AC 0.3%, f).
Kidney International  , DOI: ( /sj.ki )
Copyright © 2008 International Society of Nephrology Terms and Conditions

4. Figure 3
Mineral content ((a) calcium, (b) phosphorus) of the stomach in sham-operated rats (Sham) and uremic rats treated with vehicle (5/6 Nx) or with calcitriol 80 ng kg−1 i.p. q.o.d. for 14 days (5/6 Nx+CTR). All groups are shown with normal pH and with acidosis, which was induced by administration of NH4Cl in drinking water at 0.75% (Sham+AC 0.75%), 0.5% (5/6 Nx+AC 0.5% and 5/6 Nx+CTR+AC 0.5%), or 0.3% (5/6 Nx+CTR+AC 0.3%). aP<0.01 vs 5/6 Nx+CTR, bP<0.05 vs 5/6 Nx+CTR+AC 0.3%.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2008 International Society of Nephrology Terms and Conditions

5. Figure 4
von Kossa-stained tissue sections of the kidney and the stomach of uremic rats (5/6 Nx) treated with calcitriol, 80 ng kg−1 i.p. q.o.d. for 14 days with normal pH (5/6 Nx+CTR) and with acidosis, 0.5% NH4Cl (5/6 Nx+CTR+AC 0.5%).
Kidney International  , DOI: ( /sj.ki )
Copyright © 2008 International Society of Nephrology Terms and Conditions

6. Figure 5
In vitro studies of calcification using bovine VSMC incubated at normal (7.4) or low (7.2) pH with or without β-glycerophosphate (5 mM). (a) Calcium content; (b) Alizarin red stain.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2008 International Society of Nephrology Terms and Conditions

7. Figure 6 Experimental design of the in vivo studies.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2008 International Society of Nephrology Terms and Conditions