1. Volume 70, Issue 2, Pages 283-297 (July 2006)
The endogenous immune response modulates the course of IgA-immune complex mediated nephropathy 
T.-K. Chao, A. Rifai, S.-M. Ka, S.-M. Yang, H.-A. Shui, Y.-F. Lin, H.-K. Sytwu, W.-H. Lee, J.T. Kung, A. Chen 
Kidney International 
Volume 70, Issue 2, Pages (July 2006)
DOI: /sj.ki
Copyright © 2006 International Society of Nephrology Terms and Conditions

2. Figure 1
Comparison of the glomerular immune deposits composition associated with IgA+PnC in BCD and WT mice. Representative immunofluorescence micrographs of IgA, IgG, IgM, and C3 in the kidneys at day 14 treatment of (a–d) BCD and (e–h) WT mice with IgA+PnC are compared to (i–l) BCD mice and (m–p) WT mice that received PnC only. Original magnification × 400. (q) Semiquantitative analysis of the immunofluorescence intensity in renal tissues of the experimental groups of BCD and WT mice treated with IgA+PnC. *P<0.05; **P<0.005.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

3. Figure 2
The subclass isotypes of IgG immune deposits associated with IgA+PnC in WT mice. (a) Representative immunofluorescence micrographs of IgG1, IgG2b, and IgG3 in the kidneys at day 14 treatment of WT mice with IgA+PnC are compared to normal controls. Original magnification × 400. (b) Semiquantitative analysis of the immunofluorescence intensity for IgG1, IgG2b, and IgG3 in renal tissues of the experimental groups of WT mice treated with IgA+PnC. *P<0.05; **P<0.005.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

4. Figure 3
Time course comparison of the glomerular histopathologic changes and renal function associated with IgA+PnC or PnC only. (a) Glomerular morphology of BCD mice and WT mice treated with IgA+PnC or PnC alone on day 0 (normal), day 3, day 7, and day 14 of treatment. Original magnification × 400. (b) Semiquantitative analysis of cellular proliferation, glomerulosclerosis, cellular necrosis, and crescent formation in glomeruli of BCD and WT mice treated with IgA+PnC or PnC only. Influence of IgA+PnC or PnC alone on renal function as assessed by measurement of (c) blood urea nitrogen and (d) urinary protein.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

5. Figure 4
Lack of glomerular injury in B-and T-cell deficient (RAG-1) mice compared to immunocompetent WT controls. (a) Glomerular morphology of RAG-1 mice and WT mice treated with IgA+PnC on day 14 of treatment. Original magnification × 400. (b) Semiquantitative analysis of cellular proliferation in glomeruli of RAG-1 mice and WT controls B6.129SF2 mice treated with IgA+PnC on day 0 (normal) and day 14 of treatment. **P<0.005.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

6. Figure 5
Activation of B cells (CD19+/CD69+) by PnC and its modulation by IgA anti-PnC. (a) Immunofluorescence dot plot pattern of CD69 activation marker on CD19+ B cells in BCD mice and WT mice treated with IgA+PnC or PnC alone on day 0 (normal), day 3, day 7, and day 14. X-axis: FITC intensity of CD19. Y-axis: PE intensity of CD69. (b) Percentage of CD19+/CD69+ B cells in BCD mice and WT mice treated with IgA+PnC or PnC alone.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

7. Figure 6
Activation of T cells (CD3+/CD69+) by PnC and its modulation by IgA anti-PnC. (a) Immunofluorescence dot plot pattern of CD69 activation marker on CD3+ T cells in BCD mice and WT mice treated with IgA+PnC or PnC alone on day 0 (normal), day 3, day 7, and day 14. X-axis: FITC intensity of CD3. Y-axis: PE intensity of CD69. (b) Percentage of CD3+/CD69+ T cells in BCD mice and WT mice treated with IgA+PnC or PnC alone.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

8. Figure 7
Time course of glomerular macrophage infiltration in response to treatment with PnC only or IgA+PnC. (a) Representative immunohistochemical detection of CD11b cells in BCD mice and WT mice treated with PnC alone on day 0 (normal), day 3, day 7, and day 14 or IgA+PnC on day 14. Original magnification × 400. (b) Score of infiltrating CD11b+ cells into glomeruli in BCD mice and WT mice after the injection of PnC alone on day 0 (normal), day 3, day 7, and day 14. (c) Score of glomerular CD11b+ cells and PMNs on day 14 in BCD mice and WT mice treated with IgA+PnC or PnC alone. *P<0.05.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

9. Figure 8
Periglomerular and interstitial T-cell infiltration. (a) Representative CD3 immunohistochemical staining with antibody to CD3 in BCD mice and WT mice treated with IgA+PnC or PnC alone for 14 days and normal. Original magnification × 400. (b) Counting of infiltrating CD3+ T cells into periglomerulus and interstitium in BCD mice and WT mice after the injection of IgA+PnC on normal and day 14 of nephritis. **P<0.005.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

10. Figure 9
IL-6 and MCP-1 response to treatment with IgA+PnC in BCD mice and WT mice. (a) Serum level of IL-6 as determined by ELISA. (b) Gene expression of IL-6 in total renal tissue as determined by RT-PCR. (c) Real-time PCR of IL-6 analysis of isolated glomeruli. (d) Detection of IL-6 in glomeruli at day 14 after IgA+PnC. Original magnification × 400. (e) Serum level of MCP-1 in response to IgA+PnC. (f) Gene expression of MCP-1 in total renal tissue. (g) Real-time RT-PCR analysis of MCP-1 in isolated glomeruli. (h) Representative immunohistochemical detection of MCP-1 in experimental (day 14) and normal mice. Original magnification × 400. *P<0.05; **P<0.005.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions

11. Figure 10
Activation of NF-κB in the glomeruli associated with IgA+PnC immune deposition. (a) Representative immunohistochemical detection of Phospho-NF-κB/p65 immunostaining in glomeruli. The sections from BCD mice and WT mice on day 0 (normal), day 3, day 7, day 14 treated with IgA+PnC or PnC alone was probed with phospho-NF-κB/p65 antibody. Original magnification × 400. (b) Semiquantitative analysis of glomerular phospho-NF-κB/p65+ cells in BCD mice and WT mice treated with IgA+PnC or PnC alone.
Kidney International  , DOI: ( /sj.ki )
Copyright © 2006 International Society of Nephrology Terms and Conditions