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Diagnosis of Human Congenital Cytomegalovirus Infection by Amplification of Viral DNA from Dried Blood Spots on Perinatal Cards  Lori Scanga, Shu Chaing,

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Presentation on theme: "Diagnosis of Human Congenital Cytomegalovirus Infection by Amplification of Viral DNA from Dried Blood Spots on Perinatal Cards  Lori Scanga, Shu Chaing,"— Presentation transcript:

1 Diagnosis of Human Congenital Cytomegalovirus Infection by Amplification of Viral DNA from Dried Blood Spots on Perinatal Cards  Lori Scanga, Shu Chaing, Cynthia Powell, Arthur S. Aylsworth, Lizzie J. Harrell, Nancy G. Henshaw, Chris J. Civalier, Leigh B. Thorne, Karen Weck, Jessica Booker, Margaret L. Gulley  The Journal of Molecular Diagnostics  Volume 8, Issue 2, Pages (May 2006) DOI: /jmoldx Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

2 Figure 1 Real-time PCR of CMV DNA extracted and amplified from Guthrie cards reveals that the assay is sensitive and linear. A: Amplification plot shows change in fluorescence on the y axis (ΔRn) and PCR cycle number on the x axis. Standards contain varying levels of CMV DNA as follows: C1, 80,000 copies/5 μl blood; C2, 8000 copies/5 μl blood; C3, 800 copies/5 μl blood; C4, 80 copies/5 μl blood; C5, 8 copies/5 μl blood. B: Standard curve shows cycle threshold (Ct) on the y axis and template DNA level on the x axis. Correlation coefficient (R2) = Linearity drops off with the highest dilution (C5). The Journal of Molecular Diagnostics 2006 8, DOI: ( /jmoldx ) Copyright © 2006 American Society for Investigative Pathology and Association for Molecular Pathology Terms and Conditions

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