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Evidence for expression of eosinophil-associated IL-12 messenger RNA and immunoreactivity in bronchial asthma  Esra Nutku, MDa, Abdelilah Soussi Gounni,

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Presentation on theme: "Evidence for expression of eosinophil-associated IL-12 messenger RNA and immunoreactivity in bronchial asthma  Esra Nutku, MDa, Abdelilah Soussi Gounni,"— Presentation transcript:

1 Evidence for expression of eosinophil-associated IL-12 messenger RNA and immunoreactivity in bronchial asthma  Esra Nutku, MDa, Abdelilah Soussi Gounni, PhDa, Ron Olivenstein, MDb, Qutayba Hamid, MD, PhDa  Journal of Allergy and Clinical Immunology  Volume 106, Issue 2, Pages (August 2000) DOI: /mai Copyright © 2000 Mosby, Inc. Terms and Conditions

2 Fig. 1 A, A representative example of IL-12 (p40) mRNA in peripheral blood eosinophils from an asthmatic individual. Positive hybridization signals are localized as silver grains (magnification 200×). B, Immunofluorescent staining with an FITC-conjugated antibody against both subunits of IL-12 is shown in peripheral blood eosinophils from asthmatic subjects (magnification 1000×). C, Control experiment (no staining). D, Double-staining experiments to colocalize IL-5 and IL-12 in peripheral blood eosinophils. Arrows indicate the positive double staining. Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

3 Fig. 2 A , Mean percentages of p35 and p40 mRNA+ eosinophils in peripheral blood taken from asthmatic subjects (n = 7) and nonasthmatic control subjects (n = 7). There were statistically significantly greater percentages of IL-12 mRNA+ (both p35 and p40) cells in nonasthmatic subjects compared with asthmatic subjects. B , IL-12 immunoreactivity in peripheral blood eosinophils obtained from asthmatic subjects (n = 6), nonasthmatic control subjects (n = 7), and patients with chronic bronchitis (n = 7). The percentage of eosinophils expressing IL-12–immunoreactive protein was lower in asthmatic subjects compared with that found in patients with chronic bronchitis (P < .001) and healthy control subjects (P < .05). Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

4 Fig. 3 A representative graph demonstrating the percentages of eosinophils positive for IL-12 and IL-5 in asthmatic and control subjects (P < .001). Journal of Allergy and Clinical Immunology  , DOI: ( /mai ) Copyright © 2000 Mosby, Inc. Terms and Conditions

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