1. Volume 59, Issue 2, Pages 425-433 (February 2001)
Functional glucocorticoid receptors in the mesonephros of the ovine fetus 
Arianne Peers, Vicky Hantzis, Miodrag Dodic, Irene Koukoulas, Angela Gibson, Rebecca Baird, Renato Salemi, E. Marelyn Wintour 
Kidney International 
Volume 59, Issue 2, Pages (February 2001)
DOI: /j x
Copyright © 2001 International Society of Nephrology Terms and Conditions

2. Figure 1
Effects of corticosteroid treatment on allantoic fluid osmolality, Na, Cl, and urea concentrations of 28-day ovine fetuses. Pregnant ewes were treated for 48 hours between 26 and 28 days of gestation with either saline (S, N = 7; □) dexamethasone (D, N = 8; ▪), cortisol (C, N = 7; ), or aldosterone (A, N = 7; ). Results are means ± SEM. *P < 0.05; **P < 0.01; ***P <
Kidney International  , DOI: ( /j x)
Copyright © 2001 International Society of Nephrology Terms and Conditions

3. Figure 2
Effects of corticosteroid treatment on allantoic fluid glucose, fructose, potassium, and lactate concentrations of 28-day ovine fetuses. Pregnant ewes were treated for 48 hours between 26 and 28 days of gestation with either saline (S, N = 7), dexamethasone (D, N = 8), cortisol (C, N = 7), or aldosterone (A, N = 7). Results are means ± SEM. *P < 0.05; **P < 0.01; ***P <
Kidney International  , DOI: ( /j x)
Copyright © 2001 International Society of Nephrology Terms and Conditions

4. Figure 3
Relative quantity of GR mRNA. Pregnant ewes were infused for 48 hours between 25 and 27 days with synthetic [dexamethasone (D; ▪) 0.48 mg/hour] or natural [cortisol (F; ) 5 mg/hour; aldosterone (A; ) 10 μg/hour] corticosteroids. Isotonic saline was used as a control (□). GR was quantitated via real-time PCR. GR mRNA from 27-day ovine fetuses is expressed as a ratio to nontreated adult ovine kidney (mean ± SEM). The GR mRNA expression level was significantly down regulated (or reduced) by the administration of aldosterone. *P < 0.05.
Kidney International  , DOI: ( /j x)
Copyright © 2001 International Society of Nephrology Terms and Conditions

5. Figure 4
Bright- and dark-field photomicrographs of different regions of the 28 day fetus (A→F) and the allantoic membrane from the 28 day fetus (G and H) hybridized with the GR riboprobe. The eye region of the fetus (A and B) shows strong labeling in retinal cells and epithelial cells surrounding the eye and lower amounts of GR mRNA in muscle and capillary endothelium. (Bars = 50 μm). The mesonephros at this age (C and D) shows GR mRNA expression greatest in the proximal and distal tubules, with lower amounts seen in the collecting duct epithelium (Bars = 50 μm). Probe specificity is also confirmed by the labeling pattern seen in the 28 day fetal liver (E and F). Here, hepatocytes show expression of GR mRNA, whereas the erythroid cells are negative (bars = 100 μm). Finally, the allantoic membrane at this stage of gestation (G and H) strongly expresses GR mRNA in epithelial cells. The sense probe (insert) shows no detectable specific labeling (A, C, E, and F).
Kidney International  , DOI: ( /j x)
Copyright © 2001 International Society of Nephrology Terms and Conditions