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A dedicated automated system for extraction, quantification and STR amplification of forensic evidence samples  James Stray, Vivian T. Nguyen, Jacquelyn.

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Presentation on theme: "A dedicated automated system for extraction, quantification and STR amplification of forensic evidence samples  James Stray, Vivian T. Nguyen, Jacquelyn."— Presentation transcript:

1 A dedicated automated system for extraction, quantification and STR amplification of forensic evidence samples  James Stray, Vivian T. Nguyen, Jacquelyn Benfield, Rixun Fang, Maxim Brevnov, Lynda Treat-Clemons, Greg Porter, Manohar R. Furtado, Jaiprakash G. Shewale  Forensic Science International: Genetics Supplement Series  Volume 2, Issue 1, Pages (December 2009) DOI: /j.fsigss Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

2 Fig. 1 Total yield of DNA extracted from dried stain of 5μL blood on cotton cloth (5mm punch) from 6 human donors. The DNA was extracted using the PrepFiler™ Automated Forensic DNA Extraction kit and human DNA was quantified using the Quantifier® Human DNA quantification kit on the HID EVOlution™ Combination System. 1.5mL tubes were used for lysis as well as collection of extracted purified DNA. Forensic Science International: Genetics Supplement Series 2009 2, 64-65DOI: ( /j.fsigss ) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions

3 Fig. 2 Average peak height of alleles in the Identifiler® profile. DNA extracts with indicated concentration were quantified using Quantifier® Human DNA quantification kit, the samples were normalized to 0.1ng/μL when necessary and amplified using the Identifiler® kit. All operations were performed using the HID EVOlution™ Combination System. 1.5mL tubes were used as source container. Each concentration of DNA was run in five replicates. N indicates total number of alleles in each data set. Forensic Science International: Genetics Supplement Series 2009 2, 64-65DOI: ( /j.fsigss ) Copyright © 2009 Elsevier Ireland Ltd Terms and Conditions


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