1. Quiz#5 LC710 11/16/11 name___________
Q1:Given the following eukaryotic gene A: 1 2 3 4
gene A normally blocks mRNA translation. You want to make a lot of GENE A protein in bacteria.
Draw out and annotate your construct (insert only): it is a given that your plasmid has an
Origin of replication and a resistance marker. 4pts. Inaccuracies may incur deductions.
Let’s say that you don’t want to include exon 1, then how will your construct differ? 2pts.
Be Specific!
Let’s say that you don’t want to include exon 2, then how will your construct differ? 2pts.
Be Specific!