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Identification of multiple genes with altered expression at the distal anastomosis of healing polytetrafluoroethylene grafts  Juan A. Cordero, MD, William.

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Presentation on theme: "Identification of multiple genes with altered expression at the distal anastomosis of healing polytetrafluoroethylene grafts  Juan A. Cordero, MD, William."— Presentation transcript:

1 Identification of multiple genes with altered expression at the distal anastomosis of healing polytetrafluoroethylene grafts  Juan A. Cordero, MD, William C. Quist, MD, PhD, Allen D. Hamdan, MD, Matthew D. Phaneuf, BS, Mauricio A. Contreras, MD, Frank W. LoGerfo, MD  Journal of Vascular Surgery  Volume 28, Issue 1, Pages (July 1998) DOI: /S (98) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

2 Fig. 1 Carotid interposition prosthetic graft model. Intervening normal artery and distal anastomotic artery segments from three separate animals were used in differential display study. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

3 Fig. 2 Differential mRNA display and Northern blot analysis of two cDNA fragments that are expressed only at the distal anastomosis of our model. A, Differential display (top) and Northern blot analysis (bottom) of a cDNA fragment with sequence homology to α1 (III) collagen. B, Differential display (top) and Northern blot analysis (bottom) of a novel cDNA fragment. Northern blot analysis was performed with 20 μg of total RNA per lane from normal artery (C) and anastomotic artery segment (A) of three separate animals. The same blots were reprobed with 36B4 as a lane-loading control. Relative positions of 28S and 18S ribosomal RNA are indicated. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

4 Fig. 3 Immunohistocytochemistry of distal anastomotic artery segment performed with antibody to type III collagen. The PTFE graft is seen on the left, and the native vessel is on the right. Moderate staining (copper) is seen in the media of the vessel, and significantly enhanced staining is seen in the developing pseudointima (arrow). Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

5 Fig. 4 Expression of 2C3 and 2C5 clones in human adult tissues. A, Northern blot analysis of 2C3 cDNA clone in human adult tissues. B, Northern blot analysis of 2C5 cDNA clone in human adult tissues. The RNA blot was hybridized with 32P-labeled 2C3 and 2C5 probes followed by hybridization with β-actin as a lane-loading control. Journal of Vascular Surgery  , DOI: ( /S (98) ) Copyright © 1998 Society for Vascular Surgery and International Society for Cardiovascular Surgery, North American Chapter Terms and Conditions

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