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Extension of the Legionella pneumophila sequence-based typing scheme to include strains carrying a variant of the N-acylneuraminate cytidylyltransferase.

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Presentation on theme: "Extension of the Legionella pneumophila sequence-based typing scheme to include strains carrying a variant of the N-acylneuraminate cytidylyltransferase."— Presentation transcript:

1 Extension of the Legionella pneumophila sequence-based typing scheme to include strains carrying a variant of the N-acylneuraminate cytidylyltransferase gene  M. Mentasti, A. Underwood, C. Lück, N.A. Kozak-Muiznieks, T.G. Harrison, N.K. Fry  Clinical Microbiology and Infection  Volume 20, Issue 7, Pages O435-O441 (July 2014) DOI: / Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

2 FIG. 1 Comparison of the QV20+ values of the neuAh trace files obtained from the Legionella pneumophila serogroup 5 Dallas-1E strain (NCTC 11405) with the Farhat et al. [5] primers (left) and the novel primers (right). Clinical Microbiology and Infection  , O435-O441DOI: ( / ) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

3 FIG. 2 Schematic representation of the blastn analysis of the amplicons obtained with the primers neuA_L2 and neuAh_R2, and comparison with part of the lipopolysaccharide (LPS) locus of the Paris strain of Legionella pneumophila. Clinical Microbiology and Infection  , O435-O441DOI: ( / ) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions

4 FIG. 3 ClustalW analysis of the 50 neuA allele variants and the 22 neuAh allele variants so far described. Clinical Microbiology and Infection  , O435-O441DOI: ( / ) Copyright © 2014 European Society of Clinical Infectious Diseases Terms and Conditions


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