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Distribution of HHQ, PQS, and NHQ and total counts (TC) in a 200- by 200-μm area of a P. aeruginosa biofilm grown on an ITO-coated glass substrate (A). Distribution of HHQ, PQS, and NHQ and total counts (TC) in a 200- by 200-μm area of a P. aeruginosa biofilm grown on an ITO-coated glass substrate (A). Depth profiling was carried out so that 3D images could be reconstructed. The equivalent x-y images generated from the 3D reconstruction are shown in panel B, and virtual sections of 10 μm through the biofilm at an area with a high PQS concentration (indicated by a dashed line in panel B) are shown in panel C. Note that the z axis is shown on a relative scale (number of scans) rather than an absolute-distance scale. Sarah K. Davies et al. mSphere 2017; doi: /mSphere
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