1. Structure of a Ubiquitin E1-E2 Complex: Insights to E1-E2 Thioester Transfer 
Shaun K. Olsen, Christopher D. Lima 
Molecular Cell 
Volume 49, Issue 5, Pages (March 2013)
DOI: /j.molcel
Copyright © 2013 Elsevier Inc. Terms and Conditions

2. Figure 1 E1/Ub/ATP⋅Mg and E1-E2/Ub/ATP⋅Mg Structures
(A) Cartoon of the Uba1/Ub/ATP⋅Mg complex with Uba1 domains color coded and labeled. E1 catalytic cysteine and ATP are shown as CPK-colored spheres and magnesium ions as cyan spheres. AAD and IAD stand for active and inactive adenylation domains, respectively; FCCH is first catalytic cysteine half domain.
(B) Chemical structures of Ub/Ubl during E1-E2 thioester transfer (left) and the E1-E2 disulfide-linked complex (right). Active-site cysteine residues, E1, E2, and Ub/Ubl are labeled.
(C) Three views of a cartoon representation of the Uba1-Ubc4/Ub/ATP⋅Mg complex labeled and colored as in (A). The disulfide bridge linking E1 and E2 cysteines is indicated by an arrow. A front view of the structure shown in the left panel, a top/down view in the center panel, and a side view in the right panel.
See also Figure S1.
Molecular Cell  , DOI: ( /j.molcel )
Copyright © 2013 Elsevier Inc. Terms and Conditions

3. Figure 2 Conformational Changes Bring E1 and E2 Active Sites Together
(A) Model of a Ub E1/E2 complex with a distal UFD conformation generated by docking of Ubc4 onto the UFD of the Uba1/Ub/ATP⋅Mg structure reveals a 25 Å separation between E1 and E2 active sites. A loop in the Uba1 Cys domain that covers the E1 catalytic cysteine (Cys cap; colored cyan) is disordered in the Uba1-Ubc4/Ub/ATP⋅Mg structure.
(B) UFD conformations indicating a path from distal to proximal by superposition Uba1-Ubc4/Ub/ATP⋅Mg (red; proximal), Uba1/Ub/ATP⋅Mg (red; distal), and S. cerevisiae Uba1 (light blue; dark blue) in intermediate conformations (left panel). UFD rotation from distal to proximal breaks intramolecular contacts between the β hairpin and UFD core (right panel). “apo” refers to E1 in the absence of E2.
(C) UFD (red) as in (B) showing E2 (green) docked onto UFDs based on UFD/Ubc4 interactions in the Uba1-Ubc4/Ub/ATP⋅Mg structure. E1 adenylation and Cys domains are colored gray. UFDs undergo rigid-body rotation along a direct path from distal to proximal conformations to bring the E1 and E2 active sites together.
See also Figure S1.
Molecular Cell  , DOI: ( /j.molcel )
Copyright © 2013 Elsevier Inc. Terms and Conditions

4. Figure 3 Ubc4/Uba1 Cys Domain Interface
(A) Surface representation of Uba1 Cys domain and Ubc4 shown in open book representation with residues in the interface colored pink or magenta (Cys domain) and yellow or green (E2). Residues deemed most important for thioester transfer are colored magenta (Cys domain) and green (Ubc4). Right panels show stick and cartoon composite of the Cys domain (magenta) and Ubc4 (green) interface with potential hydrogen bonds as dashed lines. The region surrounding the E1 and E2 active sites (top right) and E2 contacts to the E1 crossover loop and ubiquitin (yellow; bottom right).
(B) Structure-function analysis of the Cys domain/Ubc4 interface. Uba1-Ubc4 thioester transfer assays were performed as described in the Experimental Procedures and are plotted as percent activity relative to wild-type.
(C) Structure-based sequence alignment for S. pombe Ub E2s (left) and other Uba1s (right) depicting regions in the E1 Cys domain/E2 interface. Ubc4 and Uba1 residues in direct contact are indicated by magenta and green circles, respectively, above the alignment. Positions subjected to mutational analysis are indicated by a star below the alignment. Residues in the alignment are colored black (hydrophobic), gold (charged), and silver (polar). Identities and similarities in E2 alignment are indicated to the right.
(D) Thioester transfer activities of Uba1 Cys domain mutants as in (B) with the indicated S. pombe Ub E2s.
Error bars in (B) and (D) represent ± 1 SD and were derived from three independent experiments. See also Figures S2, S3, S6, and S7.
Molecular Cell  , DOI: ( /j.molcel )
Copyright © 2013 Elsevier Inc. Terms and Conditions

5. Figure 4 Doubly Loaded Uba1-Ubc4
(A) Model of doubly loaded Uba1 in the absence of E2 indicating potential Ub(t) positions derived by superposition of E2s from available E2∼Ub thioester structures onto Ubc4 in the E1-E2 structure. Uba1 is in surface representation with domains color-coded as in Figure 1C. The catalytic cysteine (yellow sphere) and Cys cap (cyan) are labeled and indicated by arrows and Ub(t)s as ribbons. Ub(t)s that extend away from the E1/E2 active sites toward the front of Uba1 can be accommodated without steric clashes. It is unknown whether the Cys cap is ordered or disordered in the Uba1∼Ub thioester complex.
(B) Doubly loaded Ub E1/E2 model with UFD in a distal conformation. Ub(t) was derived from Ubc1∼Ub(t) (PDB 1FXT) as described in (A). Ubc4 was docked onto apo Uba1 as described in Figure 2A. Ub(t) does not clash with the E2 or E1 in the model.
(C) Doubly loaded Ub E1/E2 model with UFD in the proximal conformation. A channel formed by E1 and E2 residues leads to the active sites as highlighted in the center panel and close up in the right panel [Ub(t) colored orange and E1 and E2 residues are colored magenta and green, respectively; other components are labeled and colored gray]. The location of the FCCH loop that projects toward the channel is labeled and indicated by an arrow.
(D) Thioester transfer activity of Uba1 and mutants containing two (LiS), seven (LiM), and eleven (LiL) amino acid insertions in the FCCH loop as described in the Experimental Prodcedures. Error bars were derived from three independent experiments and represent ± 1 SD.
See also Figure S4.
Molecular Cell  , DOI: ( /j.molcel )
Copyright © 2013 Elsevier Inc. Terms and Conditions

6. Figure 5 Ubiquitin E1 UFD Interactions with E2
(A) Surface representation of the Uba1 UFD/Ubc4 interface in open book representation with residues in the interface colored orange and red (UFD) and yellow and green (E2). Residues deemed important for thioester transfer activity are red (UFD) and green (E2).
(B) Ribbon representation of the UFD/Ubc4 interface with side chains of select residues shown as sticks (left). Uba1-Ubc4 thioester transfer assays (right) were performed as described in the Experimental Procedures and are plotted as percent activity relative to the wild-type.
(C) Structure-based sequence alignment for S. pombe Ub E2s (left) and Uba1s (right) depicting regions in the UFD/E2 interface. Ubc4 and Uba1 UFD residues in direct contact are indicated by red and green circles above the alignment, respectively. Residues subjected to mutational analysis indicated by a star below the alignment. Residues in the alignment are shaded as in Figure 3C with identity and similarity of the sequences in the E2 alignment shown to the right.
(D) Thioester transfer activities of Uba1 UFD mutants as in (B) with indicated S. pombe Ub E2s.
Error bars in panels B and (D) represent ± 1 SD and were derived from three independent experiments. See also Figures S3 and S5.
Molecular Cell  , DOI: ( /j.molcel )
Copyright © 2013 Elsevier Inc. Terms and Conditions

7. Figure 6 E2-E1 UFD Interactions in Ub, SUMO and Nedd8 Pathways
(A) Cartoon representation of Ubc12/Uba3UFD (Huang et al., 2007) (left), Ubc4/Uba1UFD (middle), and Ubc9/Uba2UFD (Wang et al., 2010) (right) generated by superposition of the UFDs. Ubc4 (green), Ubc12 (blue), Ubc9 (orange), Uba1UFD (red), Uba3UFD (brown), are Uba2UFD (pink) with the E2 catalytic cysteine as a yellow sphere are shown.
(B) Structures as in (A) aligned by superposition of E2s. Structural elements within UFDs and E2s that would preclude noncognate E1-E2 interactions are labeled and indicated by dashed ovals and arrows. Individual structures are shown to the left/right of superposition for reference. Structure-based sequence alignment (bottom) of E1 and E2 elements within the E2/E1 UFD interface was derived from Figure S3. Residues in direct contact are shaded green and red for E1 and E2, respectively. The secondary structure is indicated above the alignment, and amino acid numbers are indicated to the left of the alignment.
See also Figures S3 and S5.
Molecular Cell  , DOI: ( /j.molcel )
Copyright © 2013 Elsevier Inc. Terms and Conditions

8. Figure 7 E2-E1 Cys Domain Interactions in Ub, SUMO, and Nedd8 Pathways
(A) Model of SUMO Ubc9 and SUMO E1 Uba2CysDomain derived from Uba1-Ubc4/Ub/ATP⋅Mg by superposition of E1s Uba2 (PDB 3KYC) and Uba1 encompassing adenylation and Cys domains followed by superposition of Ubc9 onto Ubc4.
(B) Model of Nedd8 Ubc12 and Nedd8 E1 derived from PDB 2NVU as described in (A). The Nedd8 E1 Cys domain is smaller than that in Ub and SUMO E1, but a large insertion in the AppBP1 is proximal to the E2. Structural elements unique to the Nedd8 E1 and predicted to form contacts with the E2 are labeled and indicated by arrows.
(C) Ub E1-E2 complex displayed in the same orientation to allow for direct comparison to (A) and (B). Structural elements unique to the Ub E1 that contact the E2 are labeled and indicated by arrows.
(D) Model of Ub E1 SUMO E2 (Ubc9) created by superposition of Ubc9 to Ubc4 in the Uba1-Ubc4 structure depicting clashes between Ubc9 Y134 and the Uba1 hydrophobic patch. Select residues are labeled and shown as sticks.
(E) Model of Ub E1 Nedd8 E2 created by superposition of Ubc12 to Ubc4 from the Uba1-Ubc4 structure depicting clashes between Ubc12 K147 and the Uba1 hydrophobic patch.
(F) Ub E1-E2 complex displayed in the same orientation to allow for direct comparison to (D) and (E).
(G) Structure-based sequence alignment of E1 and E2 elements within the E2/E1 Cys domain interface derived from Figure S3. Residues in direct contact are shaded green and magenta for E1 and E2, respectively. The secondary structure indicated above the alignment, and amino acid numbers are indicated to the left of the alignment.
See also Figures S3, S6, and S7.
Molecular Cell  , DOI: ( /j.molcel )
Copyright © 2013 Elsevier Inc. Terms and Conditions