1. Title: Biology 4/23/07 Objectives: Class Topics
4/29/2019
Title: Biology 4/23/07
Objectives:
To learn about DNA fingerprinting.
Class Topics
Complete video “Harvest of Fear”
GM drugs
Cloning
DNA fingerprinting
"Far better it is to dare mighty things, to win glorious triumphs even though checkered by failure, than to rank with those poor spirits who neither enjoy nor suffer much because they live in the gray twilight that knows neither victory nor defeat."
Theodore Roosevelt
Monday, April 29, :39 AM

2. Class Assignments Chapter 11 Quiz/Essay 4/27/07 Due this class period
4/29/2019
Class Assignments
What
By When
Chapter 11 Quiz/Essay 4/27/07
Due this class period
Due next class period
Due in the future

3. 4/29/2019
Grade Sheet
2A – p. 157 (5 pts.)

4. Essay possibilities Introduction 4 body paragraphs Conclusion
4/29/2019
Essay possibilities
Introduction
What are you going to cover?
Limit pronouns
4 body paragraphs
1. What is a GMO?
2. How are GMOs made?
3 & 4 opinion statements (on next slide)
Conclusion
What did you cover?

5. Body paragraphs 3 and 4 Opinion statements on…
4/29/2019
Body paragraphs 3 and 4
Opinion statements on…
What do you think the government should do about potential resistant weeds or insects due to excessive use of Bt or Round up Ready crops?
Do you think GM foods should be labeled?
Do you think GM crops should be used in developing countries?
Do you think companies, like Monsanto, should own (holds patents) genes and sell them
3rd paragraph should be supporting your opinion with facts
4th paragraph should be supporting opinion against arguments

6. Drugs GM drugs Clotting factor proteins for hemophilia
4/29/2019
Drugs
GM drugs
Clotting factor proteins for hemophilia
Erythropoetin – for anemics
Human growth hormone
Insulin - diabetics

7. From: http://esg-www.mit.edu:8001/esgbio/rdna/rdna.html#overview
4/29/2019
From:

8. Cloning Dolly the sheep in 1996
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Cloning
Dolly the sheep in 1996
Egg cell extracted from sheep and enucleated
Mammary cell extracted from Dolly
The two cells were shocked to stimulate fusion of the cells
Grown in culture
Implanted in uterus of sheep
Cloned sheep was born
Cloned animals

9. 4/29/2019

10. From: http://www.sciencecases.org/dog_cloning/dog_cloning_notes.asp
4/29/2019
From:

11. Quiz Review Cloning Drugs DNA fingerprint How to Technique Uses
4/29/2019
Quiz Review
Cloning
How to
Drugs
DNA fingerprint
Technique
Southern Blot
Uses
Recovering the Romanovs

12. 4/29/2019
Ethical Issues
Good or Baad?
Problems

13. DNA Fingerprinting RFLPs
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DNA Fingerprinting
RFLPs
Restriction fragment length polymorphisms
When restriction enzymes are used to cut DNA they leave it in different size lengths
The pattern left by the RFLPs after a Southern Blot technique is a DNA Fingerprint
Used commonly in criminal cases and forensics

14. Southern Blot technique
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Southern Blot technique
DNA of interest is cut by restriction enzymes
Fragments are separated by gel electrophoresis
Separates DNA by size
Smaller fragments move farther than large fragments
DNA is negatively charged, the ends of the gel are positively charged
DNA bands are transferred (blotted) onto filter paper
Radioactive tracers for visible bands

15. DNA Fingerprinting A Confounding Confusing Confectionery Crime
4/29/2019
DNA Fingerprinting
A Confounding Confusing Confectionery Crime
Recovering the Romanovs