1. Volume 2, Issue 3, Pages 390-406 (May 2009)
The Metabolic Response of Arabidopsis Roots to Oxidative Stress is Distinct from that of Heterotrophic Cells in Culture and Highlights a Complex Relationship between the Levels of Transcripts, Metabolites, and Flux 
Martin Lehmann, Markus Schwarzländer, Toshihiro Obata, Supaart Sirikantaramas, Meike Burow, Carl Erik Olsen, Takayuki Tohge, Mark D. Fricker, Birger Lindberg Møller, Alisdair R. Fernie, Lee J. Sweetlove, Miriam Laxa 
Molecular Plant 
Volume 2, Issue 3, Pages (May 2009)
DOI: /mp/ssn080
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

2. Figure 1
Redox State of roGFP2 in the Cytosol, Mitochondria, and Plastids of Arabidopsis Roots following Oxidative Stress.
(A) Degree of roGFP2 oxidation 0.5, 2, and 6 h after 60 uM menadione application (black bars, treatment; empty bars, control). The percentage of oxidized roGFP2 was calculated from the fluorescence ratio (488/405 nm) as described before (Schwarzländer et al., 2008) using calibration to the fully oxidized (100 mM H2O2) and fully reduced (10 mM DTT) probe in vivo. Each value represents the mean of six individual replicates (± SEM). The asterisk indicates significant differences to the control (t-test, p < 0.01).
(B) Ratiometric images of roGFP2 in control and treated roots (t  =  0.5 h).
(C) Time series of the fluorescence intensity ratio of roGFP2. A representative measurement is shown for each sub-cellular compartment (three replicates each). Black circles, cytosolic roGFP2; grey circles, plastidic roGFP2; open circles, mitochondrial roGFP2.
Molecular Plant 2009 2, DOI: ( /mp/ssn080)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

3. Figure 2
Scheme of Metabolite Abundance Changes after 0.5, 2, and 6 h of Menadione Treatment.
Solid arrows in the network diagram specify a single step connecting two metabolites; dashed arrows indicate at least two steps. The color code designates changed and unchanged metabolite abundances (Figure 1) as follows: green, not significantly altered; red, significantly decreased; blue, significantly increased; grey, not analyzed. Significant changes were evaluated using t-test (p < 0.05) (see Supplemental Figure 4 and Supplemental Table 1).
Molecular Plant 2009 2, DOI: ( /mp/ssn080)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

4. Figure 3
Glucosinolate Content in Control and Menadione-Treated Arabidopsis Roots.
Open symbols, control; closed symbols, menadione-treated. Values shown are averages obtained by analysis of nine root samples; error bars indicate S.D. Asterisks indicate significantly different from control (t-test, p < 0.05).
Molecular Plant 2009 2, DOI: ( /mp/ssn080)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

5. Figure 4
Time Series of 14C Accumulation after [U-14C]Glc Incorporation in Control and Menadione-Treated Arabidopsis Roots.
The metabolites of the ethanol soluble fraction were sub-fractionated into organic acids (A) and amino acids (B) and the 14C content of each fraction analyzed (closed symbol, treatment; open symbols, control). Values are means of four replicates ± SEM. Significance levels were verified using t-test (p < 0.05).
Molecular Plant 2009 2, DOI: ( /mp/ssn080)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions

6. Figure 5
Summary of Abundance Changes in Metabolites and Transcripts of Central Carbon Metabolism after 0.5, 2, and 6 h of Menadione Treatment.
The color code is as follows: red, decrease; blue, increase; green, unchanged; grey, not measured/detected. Transcripts encoding enzymes are indicated by chromosomal locus code. For clarity, only significantly altered transcripts are shown. Enzymes catalyzing each reaction are indicated by circled numbers: 1: phosphoglucose isomerase, 2: pyrophosphate fructose 6-phosphate phosphotransferase, 3: phosphofructokinase, 4: aldolase, 5: triosephosphate isomerase, 6: NAD-dependent glyceraldehyde 3-phosphate dehydrogenase, 7: phosphoglycerate kinase, 8: phosphoglycerate mutase, 9: enolase, 10: pyruvate kinase, 11: pyruvate dehydrogenase, 12: citrate synthase, 13: aconitase, 14: isocitrate dehydrogenase, 15: 2-oxoglutarate dehydrogenase, 16: succinyl-CoA ligase, 17: succinate dehydrogenase, 18: fumarase, 19: malate dehydrogenase, 20: phosphoenol pyruvate carboxylase, 21: glucose 6-phosphate dehydrogenase, 22: gluconolactonase, 23: 6-phospho gluconate dehydrogenase, 24: ribulose 5-phosphate isomerase, 25: ribulose 5-phosphate 3-epimerase.
Molecular Plant 2009 2, DOI: ( /mp/ssn080)
Copyright © 2009 The Authors. All rights reserved. Terms and Conditions