1. Volume 114, Issue 6, Pages 1211-1220 (June 1998)
Expression of transforming growth factor β receptors in normal human colon and sporadic adenocarcinomas 
Rally Eskinazi, Anne Resibois, Michal Svoboda, Marie-Odile Peny, Michael Adler, Patrick Robberecht, Jean-Luc van Laethem 
Gastroenterology 
Volume 114, Issue 6, Pages (June 1998)
DOI: /S (98)
Copyright © 1998 American Gastroenterological Association Terms and Conditions

2. Fig. 1
(A) Immunoblotting for RI on cell fractions of normal colon (NORM.) and colon tumor (TUMOR). The specific band corresponds to 55 kilodaltons. M, membrane fraction; N, nuclear fraction; C, cytoplasmic fraction. (B) Immunoblotting for RII on cell fractions with (2 μg/mL) or without (−) preincubation with the antigenic peptide. The specific band corresponds to 60 kilodaltons. Mb, membranous fraction. Lane 1, normal colon membrane fraction (N); lane 2, tumoral colon membrane fraction (T); lane 3, normal colon membrane fraction (N); lane 4, tumoral colon membrane fraction (T); lane 5, normal colon cytosolic fraction (N); lane 6, tumoral colon cytosolic fraction (T); lane 7, normal colon cytosolic fraction (N); lane 8, tumoral colon cytosolic fraction (T); and lane 9, tumoral colon membrane fraction (T).
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

3. Fig. 1
(A) Immunoblotting for RI on cell fractions of normal colon (NORM.) and colon tumor (TUMOR). The specific band corresponds to 55 kilodaltons. M, membrane fraction; N, nuclear fraction; C, cytoplasmic fraction. (B) Immunoblotting for RII on cell fractions with (2 μg/mL) or without (−) preincubation with the antigenic peptide. The specific band corresponds to 60 kilodaltons. Mb, membranous fraction. Lane 1, normal colon membrane fraction (N); lane 2, tumoral colon membrane fraction (T); lane 3, normal colon membrane fraction (N); lane 4, tumoral colon membrane fraction (T); lane 5, normal colon cytosolic fraction (N); lane 6, tumoral colon cytosolic fraction (T); lane 7, normal colon cytosolic fraction (N); lane 8, tumoral colon cytosolic fraction (T); and lane 9, tumoral colon membrane fraction (T).
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

4. Fig. 2
Thermocycling sequencing analysis of (A) the polyA tract and (B) the polyGT tract. No mutations were observed in either microsatellite-like region in any of the tumoral or normal samples.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

5. Fig. 2
Thermocycling sequencing analysis of (A) the polyA tract and (B) the polyGT tract. No mutations were observed in either microsatellite-like region in any of the tumoral or normal samples.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

6. Fig. 3
Immunostaining for the TGF-β RII in normal mucosa. The staining is located at the luminal surface and is absent in the deeper layers. The staining for TGF-β RI was similar (original magnification 260×). L, lumen.
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

7. Fig. 4
High-power magnification of a triangular neuroendocrine cell (arrowhead) in the deeper layers of the mucosa stained with the RII antibody. The same cell was stained with the RI antibody and the antichromogranin A antibody (original magnification 340×).
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

8. Fig. 5
Staining with RI antibody that was preincubated overnight with the control peptide. The field shown corresponds to that of Figure 7 and is free of immunoreactivity (original magnification 125×).
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

9. Fig. 6
RII immunoreactivity of a neoplastic cluster (T). The adjacent normal-looking mucosa is much less reactive, if at all (N) (original magnification 260×).
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

10. Fig. 7
RI immunoreactivity localized throughout the neoplastic glands (original magnification 125×).
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions

11. Fig. 8
RII immunoreactivity showing a patchy distribution of the staining between the groups of cells and within the same epithelial cluster (original magnification 240×).
Gastroenterology  , DOI: ( /S (98) )
Copyright © 1998 American Gastroenterological Association Terms and Conditions