1. CXC chemokine expression and synthesis in skeletal muscle during ischemia/reperfusion 
Hong T. Hua, MD, Hassan Al-Badawi, MD, Fateh Entabi, MD, Michael C. Stoner, MD, Rachel E. Diamond, James A. Bonheur, MD, Stuart Houser, MD, Michael T. Watkins, MD 
Journal of Vascular Surgery 
Volume 42, Issue 2, Pages (August 2005)
DOI: /j.jvs
Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

2. Fig 1
Skeletal muscle chemokine protein levels during ischemia/reperfusion. Keratinocyte derived cytokine (KC) levels (A) were not increased during ischemia or reperfusion in the ischemic or contralateral limbs. After 4 and 24 hours of reperfusion, tissue KC levels increased when compared with ischemic, sham, and contralateral limbs (*P < .001 vs sham). Macrophage inflammatory protein (MIP) levels (B) were not increased during ischemia or after 4 hours of reperfusion in ischemic or contralateral limbs. In contrast, after 24 hours of reperfusion, MIP levels were markedly increased in the reperfused (P < .001 vs all), but not the contralateral, limb.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

3. Fig 2
Serum levels of KC during ischemia/reperfusion. Serum KC levels were not increased compared with sham levels during 3 hours of ischemia alone (IA). In contrast, after 3 hrs ischemia and 4 hours of reperfusion (I/4R, *P<.01) and 3 hrs ischemia and 24 hours reperfusion (I/24R, **P<.001), there was a substantial progressive increase in serum KC levels that did correlate with increased levels of chemokines in reperfused skeletal muscle.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

4. Fig 3
Steady-state levels of chemokine messenger RNA (mRNA) in skeletal muscle ischemia/reperfusion. KC mRNA (A) levels were increased only after 4 and 24 hours of reperfusion. In contrast, macrophage inflammatory protein (MIP) mRNA levels (B) were increased only after 4 hours of reperfusion (*P < .001).
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2005 The Society for Vascular Surgery Terms and Conditions

5. Fig 4
Immunohistochemistry for tissue chemokines (original magnification, ×40). KC staining was intense throughout the reperfused muscle fibers at 24 hours, in contrast to sham tissue, in which the staining was minimal and nonspecific. Macrophage inflammatory protein 2 (MIP-2) staining was similarly intense in reperfused tissue, especially when compared with the pattern in sham tissue.
Journal of Vascular Surgery  , DOI: ( /j.jvs )
Copyright © 2005 The Society for Vascular Surgery Terms and Conditions