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HOXA10 mutations in congenital absence of uterus and vagina
Sasmira Lalwani, M.D., Hsin-hung Wu, M.D., Richard H. Reindollar, M.D., Mark R. Gray, Ph.D. Fertility and Sterility Volume 89, Issue 2, Pages (February 2008) DOI: /j.fertnstert Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions
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Figure 1 Portions of the HOXA10 gene sequence analyzed by denaturing gradient gel electrophoresis (DGGE). The top line is a summary diagram of the structure of the HOXA10 gene (24). The diagram below shows the gene structure with the positions of the three DNA fragments that include all of the protein-coding sequence. In some experiments, these fragments were digested into smaller subfragments using the indicated restriction enzymes before electrophoresis. The arrowheads pointing downward denote the positions of the indicated restriction sites. The cross-hatched regions indicate the positions of the protein-coding sequence. The black bars indicate the DNA sequences that were present in first melting domains in each DGGE experiment. The bottom lines summarize all portions of the protein-coding sequence that were within the lowest melting domains of any of the DNA fragments analyzed. The sequences present in higher melting domains, and thus not analyzed in these DGGE experiments, are indicated by the open boxes. Fertility and Sterility , DOI: ( /j.fertnstert ) Copyright © 2008 American Society for Reproductive Medicine Terms and Conditions
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